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The study was carried out in Eagle Island River, located in Mgbuosimini community, Port Harcourt, Nigeria. In this study, the concentration of Nickel (Ni) in sediments and periwinkles (Tympanotonos fuscatus) were determined from 25 sampling stations in Eagle Island River. The portion of the periwinkle that was assayed was the edible part. The assay was carried out using Atomic Absorption Spectrophotometer (AAS). The mean±SD of Ni concentrations in sediments and periwinkles were 2.77±1.05 mg/kg and 6.25±1.98 mg/kg respectively. The mean concentration of Ni was higher in periwinkles than in sediments. The correlation coefficient between Ni concentrations in sediment and periwinkle was 32.7%. Following the increasing industrial activities and waste disposal in the water body, the need to regularly determine the concentrations of this heavy metal in the water, aquatic lives and sediments is imperative in order to uphold environmental and human health protection.