Efficacy of Aloe vera Gel and Water-leaf Extracts for Removal of Egg Adhesiveness during Artificial Propagation of African Catfish (Clarias gariepinus, Burchel 1822)

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S. J. Fawehinmi
O. T. Adebayo
O. K. Gbadamosi


The paper aims to study the best immersion period and concentration of the plant extracts under study (water-leaf and Aloe vera gel) that can efficiently remove egg adhesiveness of Clarias gariepinus. Data generated were subjected to Multivariate Analysis of Variance Test, Tukey multiple range tests was used as a follow up procedure. Third order Polynomial regression analysis was then used to determine the best concentration and immersion period that neutralizes adhesiveness in eggs of C. gariepinus. The experiment was carried out at The Teaching and Research Fish Farm, Federal University of Technology, Akure, between October 2018 to December 2018. One male and one female C. gariepinus brood stock weighing 1.0 kg and 1.3 kg, respectively were used for the experiment. Three different concentrations (1, 3, and 5)% of the plant extracts were used. 2 g of urea diluted in 4 g NaCl/L of water was used as reference de-adhesion agent, while water without urea solution nor plant extracts was used as control. The fish eggs were rinsed with the solutions at different durations of 1, 3 and 5 minutes. Each concentration and rinsing time was recorded in duplicates. Results of the study showed that there were no significant differences (p=0.05) in the non-adhesive egg hatching of eggs immersed in Aloe vera gel and Urea solution. Number of non-adhesive egg and hatching increased in waterleaf extract which was significantly different (P=0.05) from eggs immersed in Aloe vera gel and water but not significantly different from urea solution. Therefore this study shows that waterleaf extract at 1% concentration and 1 minute immersion period can efficiently remove egg adhesiveness and increase hatching in C. gariepinus.

Aloe vera, Clarias gariepinus, egg adhesiveness, non-adhesiveness, waterleaf

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Fawehinmi, S. J., Adebayo, O. T., & Gbadamosi, O. K. (2019). Efficacy of Aloe vera Gel and Water-leaf Extracts for Removal of Egg Adhesiveness during Artificial Propagation of African Catfish (Clarias gariepinus, Burchel 1822). Asian Journal of Fisheries and Aquatic Research, 3(3), 1-13. Retrieved from http://journalajfar.com/index.php/AJFAR/article/view/30037
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FAO. The state of world fisheries and aquaculture. Meeting the Sustainable Development Goals. Rome. Licence: CC BY-NC-SA 3.0 IGO; 2018.

Ojutiku RO. Comparative survival and growth rate of Clarias gariepinus and Heteroclarias hatchlings fed live and frozen Daphnia. Pakistan Journal of Nutrition. 2008;7:527-529.

Adewolu MA, Adoti AJ. Effect of mixed feeding schedules with varying dietary crude protein levels on the growth and feed utilization of Clarias gariepinus (Burchell, 1822) fingerlings. J. Fish. Aquat. Sci. 2010;5:304-310.

Fagbenro OA, Adeparusi EO, Fapounda OO. Feedstuffs and dietary substitution for the farmed fish in Nigeria. In; National Workshop on fish Feed Development and Feeding Practices in Aquaculture Organized by FISON in collaboration with NIFFR and FAO Special Programme on Food Security (SPFS). New Bussa. 2003;60-72.

Eroudo ES, Nnubai C, Nwaduke FO. Haematological studies on four catfish species raised in water ponds. Nigerian Journal of Applied Ichthyology. 1993;9: 250-207.

Adebayo OT, Olarewaju OJ. Reproductive performance of African Catfish, Heterobranchus bidorsalis under different feeding regimes. Proceedings of the 6th International Symposium on Reproductive Physiology of Fish. 2000;4-9.

Abigail BW, Ward D, Duclos K, Peters M, Berlinsky DL. Surface disinfection and removal of adhesiveness from rainbow smelt eggs. North American Journal of Aquaculture. 2010;72:158-163.

Riehl R, Appelbaum S. A unique adhesion apparatus on the eggs of the catfish Clarias gariepinus (Teleostei, Clariidae). Journal of Applied Ichthyology. 1991;38: 191-197.

Asraf A, Muchlisin ZA, Siti-Azizah MN. Removal of eggs adhesiveness of African catfish Clarias gariepinus at different concentrations of urea solution. Aceh International Journal of Science and Technology. 2013;2(3):94-97.

Ekokotu PA, Nwachi OF. Use of pineapple juice in the elimination of egg stickiness in Mudfish (Heterobranchus bidorsalis). Global Journal of Bio-science and Technology. 2014;3(2):161-163.

Al-Hazzaa R, Hussein A. Stickiness elimination of Himri barbel (Barbus lutes) eggs. Turkish Journal of Fisheries and Aquatic Sciences. 2003;3:47-50.

Linhart O, Rondina, M, Gela D, Flasjshans M, Kocour M. Enzyme treatment for elimination of egg stickiness in tench (Tinca tinca L.), European catfish (Silurus glanisl.) and common carp (Cyprinus carpio). Fish Physiology and Biochemistry. 2003;28(1-4):507-508.

Ezekwe MO, Besong SA, Igbokwe PE. Beneficial influence of purslane and waterleaf supplement to human. FASEB J. 2001;16:A639.

Aja PM, Okaka ANC, Onu PN, Ibiam U, Urako AJ. Photochemical composition of Talinum triangulare (water Leaf) leaves. Pak. J. Nutr. 2010;9:527-530.

Adebayo OT. Reproductive performance of African Clariid Catfish Clarias gariepinus broodstocks on varying maternal stress. Journal of Fisheries International. 2006;1(1-2):17-20.

Zakes Z, Demska-Zakes K, Roszuk, Kowalska A. Removing adhesiveness from pikeperch eggs using tannin and proptease. Aquaculture Research. 2006; 239-249.

Rothbrd S. Notes on the induced spawning of the common carp, Cyprinus carpio a summary of work done at the fish breeding station at Gan Shmuel Israel. Fish Farmer. 1978;22:21-27.

El-Gamal AHE, El-Greisy ZA. Effect of removal of egg adhesiveness on hatchability and effect of different levels of salinity on survival and larval development in common carp, Cyprinus carpio. Journal of Applied Sciences Research. 2008;4(12): 1935-1945.

Southern Regional Agriculture Center and the Texas Aquaculture Extension Service; 2006.

Rottmann WR, Shireman VJ, Chapman AF. Techniques for taking and fertilizing spawn of fish. Southern Regional Aquaculture Center. 1991;426.

Akpoilih BU, Adebayo OT. Effect of formalin on the hatching rate of eggs and survival of larvae of the African Catfish Clarias gariepinus. J. Appl. Sci. Eniviron. 2010;14(4):31-34.

Demska-Zakes K, Zakes Z, Eoszuk J. The use of tannic acid to remove adhesiveness from pikeperch, Sander lucioperca, eggs. Aquaculture Research. 2005;36:1458-1464.

Ariole CN, Okpokwasili. The effect of indigenous probiotics on eggs hatching and larval viability of Clarias gariepinus. J. Applied Science. 2013;12:3625-4212.

Zarski D, Krejszeff S, Kucharczyk D, Palinska-Zarska K, Targonska K, Kupren K. The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch, Sander lucioperca. Aquaculture Research. 2015;46:324-334.

Thai BT, Ngo TG. Use of pineapple juice for elimination of egg stickiness of common carp (Cyprinus carpio). Asian Fisheries Science. 2004;17:159-162.

Krise WF, Bulkowski-Cummings L, Shellman A, Kraus K, Gould R. Increased walleye eggs hatch and larval survival after protease treatment of eggs. The Progressive Fish-Culturist. 1986;48(2):95-100.

Horvath L, Tamas GC, Seagrave C. Carp and fish culture. Fish News Books Blackwell Science. 2002;23-56.