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Aims: To examine the dietary effects of Prunus amygdalus dulcis seed meal on the sperm quality of male African Catfish (Clarias gariepinus).
Place and Duration of Study: The experiment was carried out at the Department of Fisheries and Aquaculture Technology Teaching and Research Farm, The Federal University of Technology, Akure for a period of 70 days.
Methodology: Five diets with crude protein of 40% were formulated with different inclusion levels of P. amygdalus dulcis seed powder. D1 (control) has 0 g of the powder, while D2, D3, D4 and D5 has 5, 10, 15 and 20 g of the P. amygdalus dulcis seed powder in 100 g of feed respectively. A total of 45 C. gariepinus were randomly distributed into 15 concrete tanks (2×2×1.5 m) at stocking density of 3 fish per tank and constant water level of 1 m was maintained in the experimental tanks. The fish were fed at 3% of body weight twice a day between 08.00-09.00 and 16.00-17.00 hours for a period of 70 days. At the end of the 70-day experiment, gonadosomatic index and reproductive indices such as milt volume, motility duration, spermatozoa count, percentage motility, percentage fertilization, percentage hatchability and percentage survival of fry were determined. The qualities of the milt were assessed by aid of microscope and by fertility tests. Histological examination of the testes of fish fed each of the diets was also carried out.
Results: Fish fed experimental diets showed significantly improved gonado-somatic index and reproductive indices over the control treatment. Higher gonado-somatic index and reproductive indices were recorded for the fish fed diet of 20 g/kg P. amygdalus dulcis seed powder compared to other experimental diets.
Conclusion: The results indicated that supplementing diets with P. amygdalus dulcis seed powder enhanced growth and improved gonadosomatic index, and reproductive indices of male C. gariepinus broodstocks which can be exploited in fish seeds production.
Pruszynski T. Effects of feeding on ammonium excretion and the growth of the African catfish fry. Czech Journal of Animal Sciences. 2003;48(3):106-112.
Rad F, Kurt GI, Bozaoulu AS. Effects of spatially localized and dispersed patterns of feed distribution on growth, size dispersion and feed conversion ratio of the African catfish; 2003.
Dada AA, Fagbohun AE. Effects of dietary supplementation of Date palm (Phoenix dactylifera) seeds on milt quality of African catfish (C. gariepinus) broodstocks. The Journal of Agricultural Sciences. 2018;13: 153-161.
Javeed AN, Rajeshwara N, Achur R, Nema K. Phytochemical screening and aphrodisiac property of Asparagus racemosus. International Journal of Pharmacy and Drug Research. 2011; 3(2):112-115.
Ramandeep S, Ashraf A, Gaurav G, Alok S, Jeyabalan G. Some medicinal plants with aphrodisiac potential: A current status. Journal of Acute Disease. 2013;179:188.
Oluyemi KA, Jimoh OR, Adesanya OA, Omotuyi IO, Josiah SJ, Oyesola TO. Effects of crude ethanolic extracts of Garcinia cambogia on the reproductive system of male wistar rats. African Journal of Biotechnology. 2007;6:1236-1238.
Dada AA, Ogunduyile OF. Effects of velvet bean Mucuna pruriens on sperm quality of African catfish, Clarias gariepinus broodstock. Journal of Fisheries and Aquatic Science. 2011;6(6):655-661.
Dada AA, Adeparusi EO. Dietary effects of two medicinal plants. Sesamum indicum and Croton zambesicus on the reproductive indices in female catfish (Clarias gariepinus) broodstock. Egyptian Journal of Aquatic Research. 2012;38:269-273.
Dada AA, Aguda O. Dietary effects of African walnut (Tetracarpidium conophorum) on the reproductive indices in male African catfish (Clarias gariepinus) broodstock. Journal of Coastal Life Medicine. 2015;3(6):471-475.
Dada AA, Ajilore VO. Use of ethanol extracts of Garcinia Kola as fertility enhancer in female catfish Clarias gariepinus broodstock. International Journal of Fisheries and Aquaculture. 2009;1:1-5.
Dada AA. Dietary Sesame Improves Reproductive Performance of Male African Giant Catfish. World Aquaculture. 2013;66-67.
Francis OM, Akinlolu AA, Kehinde OA. Assessment of bitter leaf (Vernonia amygdalina) as fertility enhancer in the giant African Catfish (Heterobranchus bidorsalis) broodstock. Academia Journal of Biotechnology. 2013;1(2):36-40.
USDA National Nutrient Database for Standard Reference. Agricultural Research Service, U.S. Department of Agriculture; 2004.
USDA National Nutrient Database for Standard Reference. Agricultural Research Service, U.S. Department of Agriculture; 2009.
Mitra SK, Muralidhar TS, Rao DRB. Experimental assessment of relative efficacy of drugs of herbal origin on sexual performance and hormone levels in alcohol exposed and normal rats. Phytotherapy Research. 1996;10:296-299.
Kornsteiner M, Wagner K, Elmadfa I. Tocopherols and total phenolics in 10 different nut types. Food Chemistry. 2006;98:381-387.
Milbury PE, Chen CY, Donikowski GG, Blumberg JB. Determination of flavonoids and phenolics and their distribution in almonds. Journal of Agricultural and Food Chemistry. 2006;54:5027- 5033.
AOAC (Association of Official Analytical Chemists) Official methods of analysis (18th Ed.). Gaithersburg, MD: Association of Official Analytical Chemists International. 2005;141.
Mims SD. Evaluation of activator solutions, motility duration and short-term storage of Paddle fish spermatozoa. Journal of World Aquaculture Society. 1991;22:224-229.
Canyurt MA, Akhan S. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal and Aquatic Sciences. 2008;8:171-175.
Rainis S, Mylonas CC, Kyriakou Y, Divanach P. Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants. Aquaculture. 2003;19:873-890.
King M. Fisheries Biology, Assessment and Management Blackwell Science, London. 1995;341.
Krause WJ. The art of examining and interpreting histologic preparation: A student handbook. Partheton Publishing Group, UK. 2001;9-10.
Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, Zghal K, Fki H, Damak J, Bahloul A. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Archives of Andrology. 2003;49(2):83-94.
Umeda F, Kato K, Muta K, Ibayashi H. Effect of vitamin E on function of pituitary- gonadal axis in male rats and human subjects. Endocrinology Journal. 1982; 29(3):287-292.
McLachlan RI, Donnell OL, Meachem SJ, Stanon DM. Identification of specific sites of hormonal regulation in spermatogenesis. Journal of Clinical Endocrinology and Metabolism. 2002;57: 149-179.
Hoffman A, Melmed S, Schlechte J. Patient guide to hyperprolactinemia diagnosis and treatment. Journal of Clinical Endocrinology and Metabolism. 2011; 96:35-36.
Jalali GR, Roozbeh J, Mohammadzadeh A, Sharifian M, Sagheb MM, Hamidian JA, Shabani S, Ghaffarpasand F, Afshariani R. Impact of oral zinc therapy on the level of sex hormones in male patients on hemodialysis. Renal Failure. 2010;32:417-419.
Cinar V, Polat Y, Baltaci A, Mogulkoc R. Effects of magnesium supplementation on testosterone levels of athletes and sedentary subjects at rest and after exhaustion. Biological Trace Element Research. 2011;140:18-23.
Wallock-Montelius L, Villanueva J, Chapin R, Conley AJ, Nguyen HP, Ames BN, Halsted CH. Chronic ethanol perturbs testicular folate metabolism and dietary folate deficiency reduces sex hormone levels in the Yucatan micropig. Biological Reproduction. 2007;76:455-465
Gopumadhavan S, Rafiz M, Venkataranganna MV, Kulkarni K, Mitra SK. Assessment of Tentex royal for sexual activity in an experimental model. Indian Journal of Clinical Practice. 2003; 10(13):23-26.
Billard R, Cosson MP. Some problems related to the assessment of spermmotility in freshwater fish. Journal of Experimental Zoology. 1992;261:122-131.
Billard RJ, Cosson L, Crim W, Suquet M. Sperm physiology and quality. In. Brood Stock Management and egg and larval quality, (eds: Bromage N, Roberts J), Blackwell Sc. Ltd. Osney mead, Oxford. 1995;25-52.
Bozkurt Y, Secer S, Bukan N, Akcay E, Tehin N. Relationship between body condition, physiological and biochemical parameters in brown trout. (Salmon trutta fario) sperm. Pakistan Journal of Biological Sciences. 2006; 9(5):940-944.
Iwamatsu T, Ishijima S, Nakashima S. Movement of spermatozoa and changes in micropyles during fertilization in medaka eggs. Journal of Experimental Zoology. 1993;57-64.
Adeparusi EO, Dada AA, Alale OV. The effects of medicinal plant (Kigelia africana) on sperm quality of African catfish Clarias gariepinus (Burchell, 1822) Broodstock. Journal of Agricultural Science. 2010; 2:193-199.
Sharma V, Thakur M, Chauhan NS, Dixit VK. Evaluation of the anabolic, aphrodisiac reproduction activity of Anacyclus Pyrethrum in Male Rats. Scientia Pharmaceutica. 2008;200:1-13.
USDA National Nutrient Database for Standard Reference. Agricultural Research Service, U.S. Department of Agriculture; 2016.
Suleiman SA, Ali ME, Zaki ZM, El-Malik EM, Nasr MA. Lipid peroxidation and human sperm motility: Protective role of vitamin E. Journal of Andrology. 1996;17: 530-537.
Muhammad Z. Effects of dietary vitamin E on male reproductive system. Asian Pacific Journal of Reproduction. 2017;6(4):145-150.