Alternations in Enzyme Activities of Clarias gariepinus Infected with Aeromonas hydrophila and Pseudomonas aeruginosa
Asian Journal of Fisheries and Aquatic Research,
Clarias gariepinus were infected with Aeromonas hydrophila and Pseudomonas aeruginosa, and blood samples were collected weekly for biochemical analysis to analyse their enzyme activities and pathogenesis for four weeks. The enzymes includes: aspartate aminotransferase (AST), alkaline phosphastase (ALP), acid phosphastase (ACP) and lactate – dehydrogenase (LDH). The fish were distributed in three different groups in triplicates as: control (C1 C2 C3), A. hydrophila (A1, A2, A3) and P. aeruginosa (P1, P2, P3). After two weeks of acclimatization, A1 – A3 were injected with 1.5 ml of 106 cfu/ml of A. hydrophila, P1-P3 were injected with 1.5 ml of 106 cfu/ml of P. aeruginosa, while C1-C3 were pathogen free. At the end of the experiment, it was observed that there was a constant increase, in the enzyme activities of the infected fish, indicating increase in virulence with respect to weeks of exposure but P. aeruginosa had higher pathogenicity compared to A. hydrophila.
- P. aeruginosa
- A. hydrophila
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