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Clarias gariepinus were infected with Aeromonas hydrophila and Pseudomonas aeruginosa, and blood samples were collected weekly for biochemical analysis to analyse their enzyme activities and pathogenesis for four weeks. The enzymes includes: aspartate aminotransferase (AST), alkaline phosphastase (ALP), acid phosphastase (ACP) and lactate – dehydrogenase (LDH). The fish were distributed in three different groups in triplicates as: control (C1 C2 C3), A. hydrophila (A1, A2, A3) and P. aeruginosa (P1, P2, P3). After two weeks of acclimatization, A1 – A3 were injected with 1.5 ml of 106 cfu/ml of A. hydrophila, P1-P3 were injected with 1.5 ml of 106 cfu/ml of P. aeruginosa, while C1-C3 were pathogen free. At the end of the experiment, it was observed that there was a constant increase, in the enzyme activities of the infected fish, indicating increase in virulence with respect to weeks of exposure but P. aeruginosa had higher pathogenicity compared to A. hydrophila.
Ukwe OIK, Edun OM, Akinrotimi OA. Aquaculture and fisheries: A recipy for job creation and health challenges. International Journal for Research under Literal Access. 2018b;1(4).
Obianeme NE, Obire O. Microbiological and physico-chemical characteristics of fish ponds in Port Harcourt. Current Studies in Comparative Education, Science and Technology. 2017;4: 126-139.
Otene BB, Ukwe OIK. Evaluation of heavy metal accumulation in water and sediment from Elechi Creek, Port Harcourt, Nigeria. International Journal of Geography and Environmental Management. 2018;4(1). ISSN: 2504-8821.
Schmdit AS, Bruun MS, Dalsaguard L, Pedersen K, Larsen JL. Occurrence of antimicrobial resistance in fish pathogenic and environmental bacteria associated with four Danish Rainbow Trout farms. Applied Environmental Microbiology. 2000;66(11):4909–4915.
Anderson DP. Novel techniques for fish disease diagnosis. In Diseases in Asian Aquaculture II. Eds Shariff M, Arther J.R. Subasinghe R. P. Fish Health Section. Asian Fisheries Society. Manillan. 1998;23–39.
Austin B, Aystin D. Bacterial fish pathogens. Disease in Farmed and wild fish. Ellish Horwood Ltd. West Sussex, England. 1987;3–350.
Khalil SA, Khalil RH, Sand TT, Safara MH. Studies on Pseudomonas septicemia among cultured Oreochomis niloticus. Journal of the Arabian Aquaculture Society. 2010;5(1).
Khalil RDH, Hana RE, Nadia B. Contribution to vibrosis in cultured eels (Anguilla anguilla). Journal of American Society. 2011;7(12):101-110.
Banerijee G, Nandi A, Ray AK. Assessment of hemolytic activity, enzyme production and bacteriocin characterization of Bacillus subtilis LRI isolate from gastrointestinal track of fish. Archives of Microbiology. 2016;199:115-124.
Nandi A, Banerjee G, Dan SK, Gosh K, Ray AKC. Potentiality of probiotic strain Baucillius sp. In Labeo rolita challenged by Aeromonas hydrophila: Assessment of oxidative stress, hemato-biochemcial parameters and mumum responses. Aquaculture Research; 2016.
Ukwe OIK, Edun OM, Akinrotimi OA. Growth and microbial indices in African Catfish (Clarias gariepinus) larvae fed formulated and commercial diets. Journal of Fisheries Science com. 2018a;12(2):001-008.
Amreuawho MO, Akinyemi AA, Ezeri GNO, Bankete OM, Takeet OVA. Pathogical study of Clarias gariepinus (Burchell, 1822). Sub-adult artificially infected with Pseudommas aerupinosa. Brazilian Journal of Aquatic Science and Technology. 2014;1805:54-70.
Fadi SE, Barakat M, Elgohary M. Biochemical studying of anabaena (Cyanobacterial) on Nile Tilapia. Alexandria Journal of Veterinary Science. 2013;3991-104.
Barton AB. Stress in fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. Integ and comp. Biology. 2002;42:517–525.
Celik ES. Blood chemistry (electrolytes, Iipoproteins and enzymes) values of black scorpion fish (Scorpaena porcus) in the Dardanelles. Turkey Journal of Biological Science. 2004;4(6):716-719.
Pari L, Amali DR. Protection role of tetrahydrocurcumim (THC) an active principle of rats. Journal of Pharmacy and Pharmatological Science. 2005;8:115-127.
Zaki MS, Sharaf NE, Osfor HM. Effect of vanadium toxicity on biochemical, haematological and clinicopathological changes in Clarias lazera present in River Nile. American – Eurasian Journal of Agricultural Environment. 2007;2:741- 745.
Steensma PD, Witzig TE. Elevated serum LDH in patients with non-Hodgkins lymphoria: Not always an ominous sign. British Journal of Haematology. 2001;107(2):1365-2141.
Najeeb Q, Azize R. Comparism of alkaline phosphate, lactate dehydrogenase and acid phosphatase levels in serum and synovial fluid between patients with Rheumatoid arthritis and Osteoarthritis. International Journal of Science and Research. 2013;4(4):1-4.
Yang J, Chen H. Serum metabolic enzyme activities and hepatocyte ultra structure of common carp after gallium exposure. Zoological Studies. 2003;42(3):455-461.
Rashannasab A, Afsharmanesh S, Rahimi R, Sharifian I. Alternations in the liver enzymatic activity of common carp, Cyprinus carpio in response to parasites. Dactylogynis spp. and Gyrodactyhis spp. Journal of Parastitic Diseases. 2016;40(4):1146-1149.
Itolmes RS, Goldberg E. Computational analyses of mammalian lactate dehyorogenase: Human, mouse, opossum and platy pus LDITS. Computational Biology and Chemistry. 2000;33(5):379-385.
Schueren F, Lingner T, George R, Haflins J, Gartner J, Thoms S. Peroxisiomal lactate delydrogenase is generated by translational read through in mammals. Elife. 2014;3:eo3640.
Alsaid M, Abuseliana AF, Daud HH, Mustapha NU, Bejo SK, Abdelhadi YM, Hardam LH. Haematological, biochemical and clinical signs changes following experimental infection of Streptococcus agalactiae in red hybrid tilapia (Oreochromus sp). Basic Research Journal of Agricultrual Science and Review. 2015;4(9):289-295.