Toxicity Effects of Brown Dried Pawpaw (Carica papaya) Leaf Extract to Fingerlings of African Catfish Clarias gariepinus
Asian Journal of Fisheries and Aquatic Research,
The acute and sub-lethal bioassay of aqueous extract of fresh pawpaw (Carica papaya) leaf on Clarias gariepinus fingerlings was investigated. The experiment was carried out at Department of Fisheries Teaching and Research Fish Farm, Modibbo Adama University of Technology Yola. At 96h static bioassay, symptoms of toxicity in the fish indicated that aqueous extract of fresh pawpaw leaf caused sub-acute effects such as altering fish behavior. These behaviors include air gulping, erratic swimming, discoloration, loss of reflex and skin peeling. These behavioral alterations were time and concentration dependent. Exposure to aqueous extract of fresh pawpaw leaf caused decrease in packed cells volume (PCV), haemoglobin (Hb), and red blood cell (RBC), mean corpuscular haemoglobin concentration (MCHC) and an increase in the mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV). It resulted in marked increase in white blood cells (WBC). Mortalities and LC50-96h values for Clarias gariepinus exposed to fresh pawpaw leaf extract was (10.9 ml/l). The mortality rates in extracts to Clarias gariepinus in sub-lethal exposure was lower than in acute concentrations. The growth rates were significantly reduced in fish exposed to sub-lethal concentrations of the fresh pawpaw leaf extract compared to the control fish (p<0.05).
- Acute toxicity
- Carica papaya
- Clarias gariepinus
How to Cite
Eno AE, Owo OI, Itam EH, Konya RS. Blood pressure depresssion by the fruit juice of Carica papaya (L.) in renal and DOCA - Induced hypertension in the rat. Phytotherapy Res. 2000;14:235-239.
Wilson RK, Kwan TK, Kwan CY, Sorger GJ. Effects of papaya seed extract and benzyl isothiocyanate on vascular contraction. J. Life Sci. 2002;71:497-507.
Lohiya NK, Mishra PK, Pathak N, Manivannan B, Jain SC. Reversible zoospermia by oral administration of the benzene chromatographic fraction of the chloroform extract of the seeds of Carica papaya in rabbits. Adv. Contracept. 1999;15:141-161.
Lohiya NK, Manivannan B, Mishra PK. Pathak N, Sriram S, Bhande SS, Panneerdoss S. Chloroform extract of Carica papaya seeds induces long-term reversible azoospermia in langur monkey. Asian Journal of Andrology. 2002;4:17-26.
Ofogba. A handbook of leaf extraction method. A Nigerian Quarterly Journal of Hospital Medicine. 1998;0189-2657.
OECD. Organisation of economic cooperation and development. Guidance document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. 2001; 34-57.
Finney DJ, Stevens WL. A table for the calculation of working probits and weights in probit analysis. Biometrika. 1948;35(1-2):191-201.
Svobodova D, Ravds J, Palackova W. Unified method of Haematological examination of fish. Research Inst. of Fish Culture and Hydrobiology. Vonnony Czechoslovakia. 1991;214-122.
Barata C, Baird DJ, Markich SJ. Influence of genetic and environmental factors on the tolerance of Daphnia magna straus to essential and non essential metals. Aquatic Toxicology. 1998;42:115-136.
Heijerick DG, Janssen CR, De-Coen WM. The combined effect of pH, hardness and dissolved organic carbon on the chronic toxicity of zinc to D. magna: Development of the surface response model. Archives Environmental Contamination and Toxicology. 2003;44:210-300.
Ayotunde EO, Offem BO. Acute and chronic toxicity of pawpaw (Carica papaya) seed powder to Nile Tilapia Oreocromis niloticus (Llinne 1757), Fingerlings. Journal of Agricultural Technology and Envinronment. 2005;1: 1- 4.
Albaiges J, Faran A, Soler M, Galiffa A, Marten P. Accumulation and distribution of biogenic and pollutant hydrocarbon, PCB and DDT in tissues of Western Mediterranean fishes. Marine Environmental Resource. 1987;22:1-18.
Moussa TA. Morphology of the accessory air-breathing organs of the teleost Clarias lazera (C&V). Journal of Morphology. 1956;98:125 -160.
Muniyan M, Veeraragghavan K. Acute toxicity of ethofenprox to the fresh water fish Oreochromis mossambicus (Peters). J. Environ. Biol. 1999;20:153-155.
Santhakumar M, Balaji M. Acute toxicity of an organophosphorus insecticide monochrotophos and its effects on behaviour of an air breathing fish, Anabas testudineus (BLOCH). J. Environ. Biol. 2000;21:21-123.
Ayuba VO, Ofojekwu PC. Acute toxicity of the Jimson’s weed (Datura innoxia) to the African catfish (Clarias gariepinus) Fingerlings. Journal of Aquatic Science. 2002;17:2-12.
Chung-Min L, Bo–Ching C, Sher S, Ming-Chaalin B, Chen-Wuing L, Bor-Cheng H. Acute toxicity and bioacummulation of arsenic in tilapia (Oreochromis mossambicus) from a blackfoot disease area of Taiwan. Environmental Toxicology. 2003;18(4):252-259.
Saleh AT, Sakr SA, Al-Sahhaf ZY, Bahareth OM. Toxicity of pyrethroid insecticide “Tetramethrin” in Albino Rat: Haematological and and biochemical effect. J. Egyptian Soc. Zool. 1998;25:35-52.
Zapata–Perez O, Sima-Alvarez R, Norena–Baroso E, Guemes J. Toxicity of sediments from Bahia de Chetumal, Mexico as assessed by hepatic EROD induction and histology in the Nile tilapia Oreochromis niloticus. Marine Environ. Res. 2000;50:345-356.
Ayotunde EO, Offem BO. Acute and chronic toxicity of pawpaw (Carica papaya) seed powder to nile tilapia Oreochromis niloticus (Linne 1757), Adult. African Journal of Biotechnology. 2008;7 (13):265-2274.
Jimoh WA. Nutritive value of sesame (Sesanum indicum) and sunflower (Helianthus annus) seed meals as dietary protein sources for Afrrican catfish. PhD Dissertation. Federal University of Technology Akure. 2012;265.
Gaafar AY, EL-manakhly EM, Solomon MK, Soufy H, Zaki SM, Mohammed SG, Hassan SM. Some pathological, biological and haematological investigation on nile tilapia (Oreachromis niloticus) following chronic exposure to editenphos pesticide. Journal of American science. 2010;6(10): 542-551.
Adeyome OK. Haematological and histological effect of cassava mill effluent in Clarias gariepinus. African Journal of Biomedical Research. 2005;8:175-183.
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