Optimization of Hormone for Artificial Breeding and Seed Production of Clarias batrachus (Linnaeus, 1758) Under Captive Condition
Asian Journal of Fisheries and Aquatic Research,
Aims: The study aimed to develop brood stock of Magur (Clarias batrachus), artificial breeding and seed production under captive condition using growth hormones in north-eastern Bangladesh.
Study Design: Fifteen pairs of brood Clarias batrachus were used in this experiment. Brood fishes were reared up to maturation for spawning operation in the farm by providing artificial diet for 4-5 months before onset of breeding season. In the present experiment, the brood fishes were induced with three growth hormones viz., Ovupin (100 mg dompridone and 0.2 mg S-GnRHa) at the rate of 1 ml.kg-1 body weight, Flash (20 mg S-GnRHa, 10 mg dompridone IP and propylene glycol) at the rate of 1 ml.kg-1 body weight and Human Chorionic Gonadotropin (HCG) hormone at the rate of 2272 IU.kg-1 body weight; respectively. The broods were injected with single injection in all treatments.
Place and Duration of Study: The present study was conducted on induced spawning of Magur (Clarias batrachus) at the Reliance Aqua Farm, Boilor, Trisal of Mymensingh district from March to August, 2016.
Methodology: Sexually mature healthy, uninjured male and female fishes were collected from the broodstock ponds for induced breeding. The fishes were transferred in the clean and germ free conditioning tanks for about 6-7 hours. During conditioning, continuous splash of water was given by keeping the male and female separate. The fishes were then weighed individually and injected intramuscularly with Ovupin, Flash and HCG hormone respectively. Only females were injected at 45° angle of the caudal peduncle. About 23-24 hours of post-injection the testes from males were dissected and mixed with stripping eggs for fertilization.
Results: The incubation period for fertilized eggs were 23-24 hours for all the treatments. Fertilization rates were 78.20±0.52, 93.09±0.61 and 81.60±0.97 % and hatching rates were 54.93±0.61, 75.77±0.47 and 64.41±0.71 % in Ovupin, Flash and HCG treatments, respectively.
Conclusion: This study revealed higher fertilization (93.09 %) and hatching (75.77 %) rate was achieved with Flash hormone. Therefore, Flash hormone could be effective for the artificial breeding and seed production of Magur (Clarias batrachus).
How to Cite
Mollah MFA. Domestication of riverine catfish Rita rita (Hamilton). Report submitted to UGC. 2005;12.
Alok D, Krishnan T, Talwar CGP, Lalit C. Induced spawning of catfish, Heteropneustes fossilis using gonadotropin releasing hormone analogue. Journal of Aquaculture. 1993;115:159- 167.
Areerat S. Clarias culture in Thailand. Aquaculture. 1987;63:355–362.
Sultana. Induced breeding of magur (Clarias batrachus). MS Thesis, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. 2013;37-42.
Saha MR, Mollah MFA, Roy PK. Growth and Survival of Clarias batrachus (Linn.) Larvae fed on Formulated Diets. Bangladesh Journal of Fisheries Research. 1998;2(2):151- 158.
Robert RJ. A handbook of diseases of cultured Clarias (pladuk) in Bangkok, Thailand. National Inland Fisheries Institute. 1982;37.
Khanom T. Study on Seed Production Technique of Indigenous Magur (Clarias batrachus), Shing (H. fossilis) and Pabda (Ompok pabda) Through Induced Breeding. MS Thesis, Bangladesh Agricultural University, Mymensingh. 2010;36.
Thomas PC, Rath SC, Mohapatra KD, Pillay TVR. Breeding and seed production of finfish and shellfish. Daya Publishing House, Delhi. 2003;121-122.
Ramaswami LS and Lakshman AB. Action of mammalian hormones on the spawning of catfish. Journal of Scientific Industry Research. 1959;18:185-191.
Thakur NK and Das P. Length weight relationship of H. fossilis (Bloch). Journal of Inland Fisheries Society of India. 1974; 6:95-96.
Nwadukwe FO, Ayinla OA, Abbey-Kalio NJ. Effects of various doses of acetone-dried powder carp pituitary extract and several changes of fecundity of Heterobranchus longifilis. In: JG Tobor and BIO Ezenwa (Editors). Proceedings of a seminar, 10th December, NIOMR, Lagos, Nigeria. 1991;40-45.
Hossain Q, Hossain MA, Parween S. Artificial breeding and nursery practices of Clarias batrachus (Linn). Scientific World. 2006; 4:32-37.
Rao JB and SJ Karamchandani. On the spawning biology of Ompok bimaculatus from Kulgarhi reservoir of Modhya Pradesh. Journal of Inland Fisheries Society of India. 1986; 18(2):40-47.
Naser MN, Shafi M, Shah MS, Barua G. Development of a new methodology on the artificial propagation of catfish Clarias batrachus (L) by influencing some physio-chemical parameters of the water. Bangladesh Journal of Zoology. 1990; 18(1):23-31.
Haniffa MA and Sridhar S. Induced spawning of spotted murrel (Channapunctatus) and catfish (H. fossilis) using human chorionic gonadotropin and synthetic hormone (Ovaprim). Indian Journal of Fisheries. 2002;15(2):40-47.
Srivastava PP, Raizada S, Dayal R, Chowdhury S, Lakhra WS, Yadav AK, Sharma P, Gupta J. Breeding and larval rearing of Asian catfish, Clarias batrachus (Linnaeus, 1758) on live and artificial Feed. Journal of Aquaculture Research and Development. 2012;3(4):1-4.
Sultana. Induced breeding of magur (Clarias batrachus). MS Thesis, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. 2013; 37-42.
Sahoo SK, Giri SS, Sahu AK. Breeding performance and egg quality of Clarias batrachus to evaluate ovatide doses (0.5, 1.0, 1.5 and 2.0 ml/kg body weight of female). Advanced Applied Scientific Research. 2005; 4(4):310- 323.
Peter RE, Lin HR and Van der kraak. Induced ovulation and spawning of cultured freshwater in China: Advances in application of GnRH analogues and dopamine antagonists. Aquaculture. 1988; 74:1-10.
Kohinoor AHM, MA Hossain, MG Hossain. Semi-intensive culture and production cost of pabda (Ompok pabda) with Rajput (Puntius gonionotus) and mirror carp (Cyprinus carpio var. specularis) in mini ponds. Bangladesh Journal of Zoology. 1997; 25(2): 129-133.
Abstract View: 88 times
PDF Download: 20 times