Tissue Specific Antioxidant Response of Cirrhinus mrigala (Hamilton, 1822) Exposed to Lead Chloride
Amna Abbas
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Andleeb Zahra
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Ammar Anwar
Department of Veterinary Sciences, University of Agriculture Faisalabad, Pakistan.
Hafeez Roy
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Umer Liaquat
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Fatima khan
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Kalleem Ullah
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Sahar Saleem
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
Filza Ghafoor *
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
In the present study, the effect of Lead chloride (PbCl2) on the peroxidase and catalase activity in the tissues (liver, kidney, gills and muscles) of Cirrhinus mrigala was studied. Cirrhinus mrigala were observed at sub-lethal concentrations viz. 1/3rd LC50 and 1/7th LC50 for 28 days. After chronic exposure fishes were dissected and sampling weekly. The activity of peroxidase enzyme was increased significantly (p<0.01) in experimental fish’s organs as compared to control group. Through metal stressed maximum peroxidase activity was found as 0.997±0.011 UmL-1, 0.676±0.016 UmL-1, 0.489±0.005 UmL-1 and 0.339±0.006 UmL-1 in liver, kidney, gills and muscles of fishes exposed to 1/3rd LC50 respectively. The catalase enzyme activity was decreased significantly in experimental fish organs as compared to control group. Minimum catalase enzyme activity was measured as 592.55±3.76 UmL-1, 577.32±8.64 UmL-1, 547.10±12.65 UmL-1 and 488.21± 28.57 UmL-1in the metal stressed liver, gills, kidney and muscles respectively, in fishes exposed to 1/3rd LC50. Catalase activity was found significantly higher in the control fish.
Keywords: Fisheries research, water quality, aquatic toxicology, Cirrhinus spp, lead-fish Interaction, antioxidants