Proximate Composition Analysis of Spirulina platensis in Lab Scale Cultivation: Prospects of Digested Rotten Guava as a Culture Media
Asian Journal of Fisheries and Aquatic Research, Volume 19, Issue 5,
An experiment was conducted to evaluate the proximate composition of Spirulina platensis and growth performance in supernatant of different concentrations (supernatant of 20, 40 and 60 rotten guavas) of digested rotten guava medium (DRGM). The growth rates in terms of optical density, dry cell weight and chlorophyll a of spirulina were varied from each other. The cell weight of Spirulina was attained a maximum of 0.818 ± 0.003 mg/L in 60% DRGM followed by 0.815 ± 0.0015 and 0.809± 0.0012 mg/L in supernatant of 20 and 40%, respectively on the 10th day of culture. Similar trend was also observed in the case of optical density of the media contained Spirulina, chlorophyll a content (mg/L), total biomass (mg/L), specific growth rates (on the basis of cell weight and chlorophyll a) and total biomass of Spirulina. Cell weight of Spirulina grown in these media had highly significant (P < 0.01) correlation with the chlorophyll a content (r = 0.746) and total biomass (r = 0.742) of Spirulina. The growth performance of Spirulina in supernatant of 60% DRGM was significantly (P < 0.01) higher than that of grown in 20% and 40% DRGM. The percentage of crude protein was found 53.35 ± 0.32% in supernatant of 40% DRGM. The crude lipid was attained of 10.15 ± 0.14% in supernatant of 60% DRGM which was significantly (P < 0.05) higher than that of grown in 20% and 40% of DGRM.
- Spirulina platensis
- proximate composition
- growth performance
- digested rotten guava medium ( DRGM )
How to Cite
Talukder M. Culture and growth performance of S. platensis in different concentrations of saline and kosaric medium. An Ms. Thesis Submitted to the Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. 2003;66.
Torxillo G, Pushparaj, Florenzano. A new procedure for obtaining pure cultures of S. maxima and S. platensis. Ann. Microbiol. 1985;135:165-173.
Peerpompiral Y, Sunithong, Promkutkaew. Cultivation and protein content of S. platensis grow in sugar cane molasses distillery slops mixed with water hyacinth compost extract. Program and abstracts. The 4th Asia-Pacific Conference on Algal Biotechnology, Hong Kong. 2000; 141.
Satter A. Culture and production of housefly larva and spirulina using poultry waste, and their use as food for catfish post-larvae, Ph. D Thesis, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. 2017;143.
Mario R, Papuzzo T, Tomaselli S. Outdoor mass culture of Spirulina maxima in sea water. Applied Microbiology and Biotechnology. 1986;24:47-50.
Cohen Z, Vonshak A. Fatty acid composition of Spirulina and Spirulina- like cyanobacteria in relation to their chemotaxonomy. Phytochemistry. 1991; 30:205.
Ruan JS, Guo BJ, Shu LH. Effect of spirulina polysaccharides on changes in white blood corpuscles induced by radiation in mice. Journal of Radiation Research Technology. 1990;8:210-213.
Kebede E, Ahlgren G. Optimum growth conditions and light utilization efficiency of Spirulina platensis, (Arthrospira fusiformis) (Cyanophyta) from lake Chitu, Ephiopia. Hydrobiologia. 1996;32:99-109.
Kato T. Chemistry of microalgae and their application to food. Food Chemistry. 1991; 8:30-35.
Horwitz W. (Editor): Official Methods of Analysis of the Association of Official Analytical Chemists. 14th Edition. Association of Official Analytical Chemists, Washington DC. USA. 1984;1018.
Vonshak A, Richmond A. Mass production of the blue green alga spirulina: An overview. Biomass. 1988;15:233-247.
Zarrouk C. Contribution a l’etude d’une cyanobacterie: Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthese de Spirulina maxima (Setchell et gardner) Geitler. Ph. D Thesis, University of Paris, France. 1996;412.
Bold HC, Wynne MJ. Introduction to the algae. Structure and Reproduction. Englewood Cliffs. New Jersey. 1978;706.
Vymazal J. Algae and Element Cycling in Wetlands. CRC Press, Inc., Boca Raton, Florida, USA. 1995;689.
Phang SM, Chu WL. University of malaya algae culture collection (UMACC). Catalogue of strain. Institute of Postgraduate Studies and Research, University of Malaya, Kualalumpur, Malaysia. 1999;77.
Zar JH. Biostatistics. Prentice-Hall Inc., Englewood Cliffs, New Jersey, USA. 1984;718.
Richmond A. Spirulina. In: Borowitzka MA, Borowitzka L. (Eds.). Microalgal Biotechnology, Cambridge U.P., Cambridge, UK. 1988;85-121.
Murugan T, Manikantavelu T, Saranraj P. Growth and bio-pigment production of three microalgal species in organic and inorganic media and determination of generation time-a comparative study. Original Research Article; 2012.
Dineshkumar R, Narendran R, Sampathkumar P. Cultivation of Spirulina platensis in different selective media. Indian Journal of Marine Science. 2016;45 (12):1749-1754.
Sukumaran P, Nulib R, Halimmon N, Simoh S, Omar H, Ismail A. Formulation of cost-effective medium using urea as a nitrogen source for Arthrospira platensis cultivation under real environment. Annual Research and Review in Biology. 2018;22(2):1-12.
Sharker MGU. Study of the culture of Spirulina platensis in various concentrations using papaya skin powder medium. MS. Thesis Submitted to the Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202. 2002;58.
Phang SM, Miah MS, Chu WL, Hashim H. Spirulina culture in digested sago starch factory waste water. Journal of Applied Phycology. 2000;12: 395-400.
Habib MAB, Yusoff FM, Phang SM, Mohamed S.: Growth and nutritional values of Moina micrura fed on Chlorella vulgaris grown in digested palm oil mill effluent. Asian Fisheries Science. 2003;16 (1-2):107-119.
Habib MAB, Kohinoor AHNM. Culture and production of house fry larvae and spirulina using poultry waste and their use as food for catfish post- larvae. Report on Advanced Research, Ministry of Education, Govt. of People Republic of Bangladesh. 2018;2: 66-70.
Abstract View: 288 times
PDF Download: 64 times