Toxicity Evaluation of Dual Fungicide SAAF (Carbendazim 12% and Mancozeb 63%) through Physiological Markers in Oreochromis mossambicus

Akankshya Baliarsingh

Department of Agriculture and Allied Sciences, C V Raman Global University, Bhubaneswar-752054, Odisha, India.

Ankita Mohanty

Department of Agriculture and Allied Sciences, C V Raman Global University, Bhubaneswar-752054, Odisha, India.

Smrutimayee Sahoo

Department of Agriculture and Allied Sciences, C V Raman Global University, Bhubaneswar-752054, Odisha, India.

Suraj Kumar Chhatoi

Department of Agriculture and Allied Sciences, C V Raman Global University, Bhubaneswar-752054, Odisha, India.

Kishan Kumar Sharma

Department of Agriculture and Allied Sciences, C V Raman Global University, Bhubaneswar-752054, Odisha, India.

Neelanjana Choudhury *

Department of Agriculture and Allied Sciences, C V Raman Global University, Bhubaneswar-752054, Odisha, India.

*Author to whom correspondence should be addressed.


Abstract

The study was designed to evaluate the acute toxicity induced by SAAF in freshwater fish, Oreochromis mossambicus. Fungicides are widely used for pest management and increase crop output. When used in excessive doses, they are also exceedingly dangerous to non-target organisms. The present investigation is about changes in biochemical parameters in Oreochromis mossambicus due to toxicity induced by the application of fungicides in paddy-cum-fish ecosystem in India. The SAAF fungicide, a mixture of carbendazim (12%) and mancozeb (63%) is used to combat fungal diseases such as sheath blight, sheath rot, blast, and leaf spot diseases of rice plants. The present research aimed to determine the toxicity and evaluate the effect of sublethal concentrations of SAAF on some biochemical, haematological, and histological parameters of Oreochromis mossambicus after 10, 20 and 30 days. Oreochromis mossambicus were exposed to different concentrations of SAAF (10.5 mg/lit, 11.5mg/lit and 12.5 mg/lit) respectively and LC50 was determined. LC50 for SAAF was detected to be 13.5mg/lit using SPSS Vs.21. After 30 days of exposure, the individuals exposed to toxicity were sacrificed and the parameters were checked in the treated fish. Among biochemical parameters, total glucose, cholesterol, stress-related enzymes AST and ALT showed a significant increase while total protein showed a substantial decrease, whereas among haematological parameters, Haemoglobin, RBC, and PCV had risen and WBC and MCV showed a marked decrease. The tissue necrosis was quite prominent in the gill, heart, liver, kidney, and muscle after 30 days of exposure to the fungicide.

Keywords: Oreochrmis mossambicus, SAAF, biochemical parameters, heamaotological parameters, histological parameters


How to Cite

Baliarsingh , Akankshya, Ankita Mohanty, Smrutimayee Sahoo, Suraj Kumar Chhatoi, Kishan Kumar Sharma, and Neelanjana Choudhury. 2023. “Toxicity Evaluation of Dual Fungicide SAAF (Carbendazim 12% and Mancozeb 63%) through Physiological Markers in Oreochromis Mossambicus”. Asian Journal of Fisheries and Aquatic Research 25 (5):28-40. https://doi.org/10.9734/ajfar/2023/v25i5697.

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References

Swaminathan MS. An evergreen revolution. Crop Science. 2006;46:2293–2303.

Jewitt S, Baker K. The green revolution re-assessed: insider perspectives on agrarian change in Bulandshahr District, Western Uttar Pradesh, India. Geoforum; J Physic Human Reg Geosci. 2005;38:73–89.

Liu Y, Pan X, Li JA. 1961–2010 record of fertilizer use, pesticide application and cereal yields: A review. Agro Sus Dev. 2015;35:83–93.

De Silva SS, Nguyeen TTT, Turchini GM, Amarasinghe US, Abery NW. Alien species in aquaculture and biodiversity: A paradox in food production. Ambio. 2009;38:24–28.

Blaylock RB, Bullard SA. Counter-insurgents of the blue revolution? Parasites and diseases affecting aquaculture and science. J Parasitol. 2014; 100:743–755.

Ahmed N, Turchini GM. The evolution of the blue-green revolution of rice-fish cultivation for sustainable food production. Sus Sci. 2021;16:1375–1390.

Ghosh Α, Saha SK, Banerjee RK, Mukherjee AB, Naskar KR. Package of practices for increased production in rice-cum-fish farming system. Aquaculture Extension Manual 4, Central Inland Fisheries Research Institute. 1985;1-14.

Alikunhi KH. Rice Field Fish Culture Outside Indonesia. International Inland Fisheries Training Centre, Bongor, Indonesia (mimeo); 1955.

Gupta MV, Sollows JD, Mazid MA, Rahman Α, Hussain G, Dey MM. Integrating aquaculture with rice farming in Bangladesh: Feasibility and economic viability, its adoption and impact. ICLARM Newsletter. 1998;1-3.

Vromant N, Chau NTH, Ollevier F. The effect of rice seeding rate and fish stocking on the floodwater ecology of the rice field in direct-seeded, concurrent rice-fish systems. Hydrobiologia. 2021;445:151–164.

Khan MZ, Francis CPL. Adverse effects of pesticides and related chemicals on enzyme and hormone systems of fish, amphibians and reptiles. Proc. Pakistan AcMad. Z Sahceie. 2005;42:15-323.

O'Brien RD. Insecticides action and metabolism Academic Press, London; 1967.

Mendel B, Kemp A, Myers DK. A colorimetric micro-method for the determination of glucose. Biochem. 1954; 56:639–646.

Tietz NW, Pruden EL, Siggard AO. Textbook of clinical chemistry. Saunders, Philadelphia; 1986.

Dumas BT. Determination of total protein in serum. Clin Chem. 1975;21:1159- 1166.

Reitman S, Frankel S. Glutamic – pyruvate transaminase assay by colorimetric method. Ame J Clin Path. 1957;28: 56.

Hesser EF. Methods for routine on fish haematology. The prog. Fish Cult. 1960; 22:164-171.

Blaxhall PC, Daisley KW. Routine hematological methods for use with fish blood. J Fish Biol. 1973;5:771-781.

Wintrobe WM. A hematological odyssey. Johns Hopkins Med. 1967;120:287s. 1926- 66.

Dacie JV, Lewis SM. Practical Haematology 5th.ed. Churchill; 1975.

Min EU, Kang JC. Effect of waterborne benomyl on the hematological and antioxidant parameters of the Nile tilapia, Oreochromis niloticus, Pest Biochem Physiol. 2008;92:138–143.

Sturve J, Almroth BC, Förlin L. Oxidative stress in rainbow trout (Oncorhynchus mykiss) exposed to sewage treatment plant effluent, Ecotoxicol Environ Saf. 2008;70:446–452.

Üner N, Oruç EÖ, Sevgiler Y, Sahin N, Durmaz H, Usta D. Effects of diazinon on acetylcholinesterase activity and lipid peroxidation in the brain of Oreochromis niloticus, Environ Toxicol Pharmacol. 2006;21:241–245.

John PJ. Alteration of certain blood parameters of freshwater teleost Mystus vittatus after chronic exposure to metasystox and sevin, Fish Physiol Biochem. 2007;33:15–20.

Monteiro, M., Quintaneiro, C., Pastorinho, M., Pereira, M. L., Morgado, F., Guilhermino, L., Soares AMVM. Acute effects of 3, 4-dichloroaniline on biomarkers and spleen histology of the common goby Pomatoschistus microps, Chemosphere. 2006;62: 1333–1339.

Velisek J, Dobsikova R, Svobodova Z, Modra H, Luskova V. Effect of deltamethrin on the biochemical profile of common carp (Cyprinus carpio L.), Bull Environ Contam Toxicol. 2006;76:992–998.

Banaee M, Mirvagefei AR, Rafei GR, Majazi Amiri B. Effect of sub-lethal diazinon concentrations on blood plasma biochemistry, Int J Environ Res. 2008;2:189–198.

Saha S, Chukwuka AV, Mukherjee D, Patnaik L, Nayak S, Dhara K, et al. Chronic effects of diazinon® exposures using integrated biomarker responses in freshwater walking catfish, Clarias batrachus. Appl. Sci. 2021;11(22): 10902.

Raja M, Al-Fatah A, Ali M, Afzal M, Hassan RA, Menon M, Dhami MS. Modification of liver and serum enzymes by paraquat treatment in rabbits. Drug Meta. Drug Inter. 1992;10:279– 291.

Das BK, Mukherjee SC. A histopathological study of carp Labeo rohita exposed to Hexachlorocyclohexane.Veterinarski Archiv. 2000;70 (4):169-180.

Jee JH, Masroor F, Kang JC. Responses of cypermethrin-induced stress in haematological parameters of Korean rockfish, Sebastes schlegeli (Hilgendorf). Aqua Res. 2005;36:898–905. DOI: 10.1111/j.1365-2109.2005.01299.x

O¨ner M, Atli G, Canli M. Changes in serum biochemical parameters of freshwater fish Oreochromis niloticus following prolonged metal (Ag, Cd, Cr, Cu, Zn) exposures. Environ Toxicol Chem. 2008;27:360–366. DOI: 10.1897/07- 281R.1

Yeragi SG, Ranaand AM, Koli VA. Effect of pesticide on protein metabolism of mudskipper, Boleophtalmus dussumieri, J Ecotoxicol Environ Monit. 2003;13:211-214.

Remia KM, Logaswamy S, Logankumar K, Rajmohan D. Effect of an insecticides (Monocrotophos) on some biochemical constituents of the fish Tilipia Mossambica. Poll. Res. 2008;27:523-526.

Pang- Hung Y, Jian S, Amartalingam R, Choon-Fah JB. Boric Acid Levels in Fresh Noodles and Fish Ball. Ame J Agri Biol Sci. 2008;3:476-481.

Palanivelu V, Vijayavel K, Ezhilarasibalasubramanian S, Balasubramanian MP. Influence of insecticidal derivative (Cartap Hydrochloride) from the marine polychaete on certain enzyme systems of the freshwater fish Oreochromis mossambicus. J Environ Biol. 2005;26: 191–196.

Rainza -Paiva MJT, Ishikawa CM, Das Eiras AA, Felizardo NN. Hematological analysis of Chara Pseudoplatystoma fasciatum in the new millennium. Nice, France, European Aquaculture Soc. Special Pub. 2000;28:590

Haley PJ, Weister MG. Erythrocyte volume distribution in rainbow trout. Ame J Vet Res. 1985;46(10):2210- 2212.

Atamanalp M, Yanik T. Alternation in haematological parametrrs of rainbow trout Oncorhyncus mykiss exposed to mancozeb, Turk. J. Vet. Anim. Sci. 2002; 27:1213-1217.

Ranganatha Koundinya P, Ramamurthi R. Haematological studies in the Sarotherodon mossambica (peters) exposed to a lethal concentration of sumithion and seven, Curr. Sci. 1980; 49(16):645-646.

Shakoori AR, Mughal AL, Iqbal MJ. Effect of sublethal doses of fenevalerate (a synthetic pyrethroid) administered continuously for four weeks on the blood, liver and muscles of fresh water fish, Ctenopharyngodon Idella. Bull Environ Contam Toxicol. 1996;3(57):487-494.

Kumar S, Lata S, Gopal K. Deltamethrin induced physiological changes in freshwater catfish Heteropneustes fossilis, Bull. Environ. Contam. Toxicol. 1998;62(3): 254-258.

Cengiz EI, Unlu E. Sub-lethal effects of commercial deltamethrin on the structure of the gill, liver and gut tissues of mosquitofish, Gambusia affinis: A microscopic study. Environ. Toxicol. Pharmacol. 2006;21:246-253.

Boran H, Altinok I, Capkin E. Histopathological changes induced by maneb and carbaryl on some tissues of rainbow trout, Oncorhynchus mykiss. Tissue Cell. 2010;42(3):158-64.

Boran H. Assessment of acute toxicity and histopathology of the fungicide captanin rainbow trout. Expt Toxicol Pathol. 2012;64(3):175-179.

Dar OI, Aslam R, Sharma S, Kaura A, Jia A, Faggio C. Biomolecular alterations in the early life stages of four food fishes following acute exposure of Triclosan. Environ. Toxicol. Pharmacol. 2022a;91: 103820.

Dar OI, Aslam R, Pan D, Sharma S, Andotra M, Kaur A, Jia A, Faggio C. Source, bioaccumulation, degradability and toxicity of triclosan in aquatic environments: A review. Environ. Technol. Innov. 2022b;25:102122.

Saha S, Kaviraj A. Effects of cypermethrin on some biochemical parameters and its amelioration through dietary supplementation of ascorbic acid in freshwater catfish Heteropneustes fossilis, Chemosphere. 2009;74:1254–1259.

Sharma S, Dar OI, Andotra M, Sharma S, Kaur A, Faggio C. Environmentally relevant concentrations of triclosan induce cyto-genotoxicity and biochemical alterations in the hatchlings of Labeo rohita. Appl. Sci. 2021a;11:10478.

Akter R, Pervin MA, Jahan H. Toxic effects of an organophosphate pesticide, envoy 50 SC on the histopathological, hematological, and brain acetylcholinesterase activities in stinging catfish (Heteropneustes fossilis). 2020; JoBAZ81: 47.

Jayachandran K, Pugazhendy K. Histopathological Changes in the Gill of Labeo rohita (Hamilton) Fingerlings Exposed to Atrazine. J Sci Res. 2009; 4(3):219-221.

Ullah R, Zuberi A, Tariq M, Ullah S. Acute Toxic Effects of Cypermethrin on Hematology and Morphology of Liver, Brain and Gills of Mahseer (Tor putitora). Int. J Agri Biol; 2014.

Santos RFB, Dias HM, Fujimoto RY. Acute toxicity and histopathology in ornamental fish amazon bluespotted corydora (Corydoras melanistius) exposed to formalin. Ann. Brazil. Acad. Sci. 2012; 84(4):1001-1007.