Orange Peel Waste Improved the Growth Performance, Feed Utilization, Oxidative Stress, and Hematological Parameters of Labeo rohita

Tuba Latif Virk

Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.

Arifa Mehreen *

Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan.

Majeeda Rasheed *

Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan.

Ghazala Naheed

Poultry Research Institute, Rawalpindi, Pakistan.

Kumbukani Mzengereza

Department of Aquatic and Fisheries Science, Mzuzu University, Private bag 201, Mzuzu, Malawi.

Mohammed F. El Basuini

Faculty of Agriculture, Tanta University, 31527, Tanta, Egypt and Faculty of Desert Agriculture, King Salman International University, South Sinai, Egypt.

Tehreem Rehman

Govt. Associate College (W), 36/SB, Sargodha, Pakistan.

Akram Ismael Shehata

Department of Animal and Fish Production, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.

*Author to whom correspondence should be addressed.


Expensive and ineffective feed ingredients in fish feed are becoming a major problem for fish growth in the aquaculture industry. Therefore, the experiment was conducted to investigate the utilization of fruit processing wastes as a dietary component in the feed of rohu (Labeo rohita). Four different feed formulations were prepared using commercial diet and supplemented with different levels of orange peel powder (CT= 0%, T1= 15%, T2= 30% and T3= 45%). The experiment was performed in aquariums of dimensions 1x1.5x2 feet, filled with approximately 79 liters of water. Fish were fed two times a day at rate of 3% of their body weight for 35 days. The results showed that the T1, T2, and T3 treatments had almost 104%, 160%, and 68% higher weight gain than that of CT, respectively. The T1 (7.55%), T2 (17.78%), and T3 (4.26%) treatments had a lower feed conversion ratio than that of CT. The T1, T2, T3 treatments had almost 14.18%, 15.27%, and 6.11% lower mean corpuscular hemoglobin concentration than that of CT. The T1 and T2 treatments had almost 7.01% and 17.42% higher total serum protein than that of CT, respectively. The T1 (0.90%) and T2 (6.23%) treatments had higher A/G ratios than that of CT, respectively and T3 (0.01%) had lower A/G ratio than that of CT. Overall, the dietary supplementation of 30% orange peel improved growth performance, feed utilization, oxidative stress, and hematological parameters in Labeo rohita.

Keywords: Feed utilization, growth performance, orange waste, oxidative stress

How to Cite

Virk, T. L., Mehreen, A., Rasheed, M., Naheed, G., Mzengereza, K., Basuini, M. F. E., Rehman, T., & Shehata, A. I. (2023). Orange Peel Waste Improved the Growth Performance, Feed Utilization, Oxidative Stress, and Hematological Parameters of Labeo rohita. Asian Journal of Fisheries and Aquatic Research, 25(5), 69–81.


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Metian M, Troell M, Christensen V, Steenbeek J, Pouil S. Mapping diversity of species in global aquaculture. Rev. Aquac. 2020;12:1090–1100.

Jahan N, Islam SMM, Rohani MF, Hossain MT, Shahjahan M. Probiotic yeast enhances growth performance of rohu (Labeo rohita) through upgrading hematology, and intestinal microbiota and morphology. Aquaculture. 2021;545.

Jobling M. Fish nutrition research: Past, present and future. Aquac. Int. 2016;24: 767–786.

Lim KC, Yusoff FM, Shariff M, Kamarudin MS. Dietary astaxanthin augments disease resistance of Asian seabass, Lates calcarifer (Bloch, 1790), against Vibrio alginolyticus infection. Fish Shellfish Immunol. 2021;114:90–101.

Md Nor S, Ding P. Trends and advances in edible biopolymer coating for tropical fruit: A review. Food Res. Int. 2020;134:109208.

Glencross BD, Baily J, Berntssen MHG, Hardy R, MacKenzie S, Tocher DR. Risk assessment of the use of alternative animal and plant raw material resources in aquaculture feeds. Rev. Aquac. 2020;12: 703–758.

Naylor RL, Hardy RW, Buschmann AH, Bush SR, Cao L, Klinge, DH, Little DC, Lubchenco J, Shumway SE, Troell M. A 20-year retrospective review of global aquaculture. Nature. 2021;591:551–563.

Felix e Silva A, Copatti CE, de Oliveira EP, Bonfá HC, Melo de FVST, Camargo AC de S, Melo JFB. Effects of whole banana meal inclusion as replacement for corn meal on digestibility, growth performance, haematological and biochemical variables in practical diets for tambaqui juveniles (Colossoma macropomum). Aquac. Reports. 2020;17:100307.

Ben-Othman S, Jõudu I, Bhat R, Beatriz M, Oliveira P, Alves RC. Bioactives from Agri-Food Wastes: Present Insights and Future Challenges. Mol. 2020; 25:510.

Maharjan M, Ulaganathan M, Aravindan V, Sreejith S, Yan, Q, Madhavi S, Wang JY, Lim TM. Fabrication of High Energy Li–Ion Capacitors from Orange Peel Derived Porous Carbon. Chemistry Select. 2017;2: 5051–5058.

Siles JA, Vargas F, Gutiérrez MC, Chica AF, Martín MA. Integral valorisation of waste orange peel using combustion, biomethanisation and co-composting technologies. Bioresour. Technol. 2016; 211:173–182.

Verma R, Singhbabu YN, Didwal PN, Nguyen AG, Kim J, Park CJ. Biowaste Orange Peel-Derived Mesoporous Carbon as a Cost-Effective Anode Material with Ultra-Stable Cyclability for Potassium-Ion Batteries. Batter. Supercaps. 2020;3: 1099–1111.

Xiao K, Liu H, Li Y, Yi L, Zhang X, Hu H, Yao H. Correlations between hydrochar properties and chemical constitution of orange peel waste during hydrothermal carbonization. Bioresour. Technol. 2018; 265:432–436.

Lee CKM, Ng KKH, Kwong CK, Tay ST. A system dynamics model for evaluating food waste management in Hong Kong, China. J. Mater. Cycles Waste Manag. 2019;21433–456.

Qiang J, Khamis OAM, Jiang HJ, Cao ZM, He J, Tao YF, Xu P, Bao JW. Effects of dietary supplementation with apple peel powder on the growth, blood and liver parameters, and transcriptome of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). PLoS One 2019; 14:e0224995.

Choi WM, Lam CL, Mo WY, Wong MH. The use of food wastes as feed ingredients for culturing grass carp (Ctenopharyngodon idellus) in Hong Kong. Environ. Sci. Pollut. Res. 2016;23:7178–7185.

NRC. National Research Council (NRC): Nutrient requirements of fish and shrimp: The National Academies Press, Washington, DC, 2011, 376+ XVI pp,£ 128 (Hardback); 2012. ISBN: 978-0-309-16338-5.

Chemists A, OA. Official Methods of Analysis of Official Analytical Chemists International, 17th Edn. J. AOAC Int. 2009; 92:61–73.

Castell JD, Tiews K. Report of the EIFAC, IUNS and ICES Working Group on Standardization of Methodology in Fish Nutrition Research, Hamburg, Federal Republic of Germany, 21-23 March 1979. Doc. Tech. la CECPI; 1980.

Chatzifotis S, Panagiotidou M, Papaioannou N, Pavlidis, M, Nengas I, Mylonas CC. Effect of dietary lipid levels on growth, feed utilization, body composition and serum metabolites of meagre (Argyrosomus regius) juveniles. Aquaculture. 2010;307:65–70.

Wootton IDP. Micro-analysis in medical biochemistry. Micro-analysis Med. Biochem; 1964.

Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 1957; 28:56–63.

Altendorf S. Minor tropical fruits mainstreaming a niche market. Food Agric. Organ. United Nations. 2018;67–74.

Pfaltzgraff LA, Cooper EC, Budarin V, Clark JH. Food waste biomass: a resource for high-value chemicals. Green Chem. 2013;15:307–314.

Singh B, Singh JP, Kaur A, Singh N. Phenolic composition, antioxidant potential and health benefits of citrus peel. Food Res. Int. 2020;132:109114.

Vicente IST, Fleuri LF, Carvalho PLPF, Guimarães MG, Naliato RF, Müller H, de C, Sartori MMP, Pezzato LE, Barros MM. Orange peel fragment improves antioxidant capacity and haematological profile of Nile tilapia subjected to heat/dissolved oxygen-induced stress. Aquac. Res. 2019;50:80–92.

Shabana MS, Karthika M, Ramasubramanian V. Effect of dietary Citrus sinensis peel extract on growth performance, digestive enzyme activity, muscle biochemical composition, and metabolic enzyme status of the freshwater fish, Catla catla. J. Basic Appl. Zool. 2019;80.

Magalhães D, Vilas-Boas AA, Teixeira P, Pintado M. The lemon as a fruit is the one that is fully exploited because the fruit is mainly used for its juice, while the fleshier part of the fruit and the peel are particularly used in culinary applications. Foods. 2023;12.

Dawood MAO, Zommara M, Eweedah NM. Helal AI. The evaluation of growth performance, blood health, oxidative status and immune-related gene expression in Nile tilapia (Oreochromis niloticus) fed dietary nanoselenium spheres produced by lactic acid bacteria. Aquaculture. 2020;515: 734571.

Cai L, Li YP, Wei ZX. Li XL, Jiang XR. Effects of dietary gallic acid on growth performance, diarrhea incidence, intestinal morphology, plasma antioxidant indices, and immune response in weaned piglets. Anim. Feed Sci. Technol. 2020; 261.

Salem M, Abdel-Ghany HM. Effects of dietary orange peel on growth performance of nile tilapia (Oreochromis niloticus) fingerlings. Aquac. Stud. 2018;18:127–134.

Doan H, Van Hoseinifar SH, Elumalai P, Tongsiri S, Chitmanat C, Jaturasitha S, Doolgindachbaporn S. Effects of orange peels derived pectin on innate immune response, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus) cultured under indoor biofloc system. Fish Shellfish Immunol. 2018;80: 56–62.

Salem MES, Abdel-Ghany HM, Sallam AE, El-Feky MMM, Almisherfi HM. Effects of dietary orange peel on growth performance, antioxidant activity, intestinal microbiota and liver histology of Gilthead sea bream (Sparus aurata) larvae. Aquac. Nutr. 2019;25:1087–1097.

United States Agency for International Development (USAID). Feed Conversion Ratio (FCR). USAID Harvest. 2011;1–2.

Boyd CE. Protein conversion efficiency in aquaculture. Glob. Aquac. Advocate. 2018;8–11.

Bowyer PH, El-Haroun ER, Salim HS, Davies SJ. Benefits of a commercial solid-state fermentation (SSF) product on growth performance, feed efficiency and gut morphology of juvenile Nile tilapia (Oreochromis niloticus) fed different UK lupin meal cultivars. Aquaculture. 2020; 523.

Oluremi OIA, Ngi J, Andrew IA. Phytonutrients in citrus fruit peel meal and nutritional implication for livestock production. Livest. Res. Rural Dev. 2007; 19:345–346.

Goda AMAS. Effect of dietary ginseng herb (Ginsana ® G115) supplementation on growth, feed utilization, and hematological indices of Nile Tilapia, Oreochromis niloticus (L.), fingerlings. J. World Aquac. Soc. 2008;39:205–214.

Virgili F, Marino M. Regulation of cellular signals from nutritional molecules: a specific role for phytochemicals, beyond antioxidant activity. Free Radic. Biol. Med. 2008;45:1205–1216.

Mayssara A Abo Hassanin Supervised A, Munawarah SH, Misnaniarti M, Isnurhadi I, Komunitas JK, Rumbai P, City P, Komitmen P, Kbpkp P, Commitment S, Kbpkp F, Dewi NM, Hardy IPD, Sugianto M, Ninla Elmawati Falabiba T, Anton Kristijono Sandra C, Herawati YT, Baroya N, Trirahayu P, Akbar KA, Ramani A, Pencapaian A, Kapitasi I, Komitmen B, Victor J, Maramis F, Mandagi CKF, Wowor R, Kesehatan F, Universitas,M, Ratulangi S, Febriawat, H, Yandrizal Y, Afriza Y, Pratiwi BA, Yanuarti R, Suryani D, Hiv K, Aids DAN, Rumah DI, Utara US, Badan Penyelenggara Jaminan Sosial (BPJS) Kesehatan, Mayssara A. Abo Hassanin Supervised A, Vii BAB, Husada FRK, Info BPJS Kesehatan, Anugrah S. BPJS-Kesehatan, Trisnantoro L, LAILY EN, Utami D. siwi Murti B, Suryani N, Kurnia R, Fadli Kemkes RI, BPJS Kesehatan Laksono T, dkk Indonesia U, Maujudah SA, Masyarakat FK, Studi P, Kesehatan I,. Effect of orange peel waste on growth performance and feed utilization of red Tilapia (Oreochromis Mossambicus X O. niloticus) Fingerlings. Pap. Knowl. Towar. a Media Hist. Doc. 2019;7:1–33.

de Moraes TCH, Ferreira CM, Gama KF, da S, Hoshiba MA, Povh JA, de Abreu JS. Routine exposure to biometric procedures in fish farming reveals differences in stress response in tambaqui and hybrid tambatinga. Bol. do Inst. Pesca. 2017;43: 1–10.

Svobodova Z, Pravda D, Palackova J Unified methods of haematological examination of fish. Research Institute of fish culture and hydrobiology; 1991.

Esmaeili M. Blood performance: A new formula for fish growth and health. Biology (Basel). 2021;10:1236.

Mukrimaa SS, Nurdyansyah Fahyuni EF, Yulia Citra A, Schulz ND, Ghassan, Taniredja T, Faridli EM, Harmianto S. Covariance structure analysis of health-related indicators for home-dwelling elderly people centered on subjective health. J. Penelit. Pendidik. Guru Sekol. Dasar 2016; 6:128.

Acar U, Kesbiç OS, Yilmaz S, Gültepe N, Türker A. Evaluation of the effects of essential oil extracted from sweet orange peel (Citrus sinensis) on growth rate of tilapia (Oreochromis mossambicus) and possible disease resistance against Streptococcus iniae. Aquaculture 2015; 437:282–286.

Humphries DL, Scott ME, Vermund SH. Pathways linking nutritional status and infectious disease: causal and conceptual frameworks. Nutr. Infect. Dis. Shifting Clin. Paradig. 2021;3–22.

Tani S, Kawauchi K, Atsumi W, Matsuo R, Ashida T, Imatake K, Suzuki Y, Yagi T, Takahashi A, Matsumoto N, Okumura Y. Association among daily fish intake, white blood cell count, and healthy lifestyle behaviors in an apparently healthy Japanese population: implication for the anti-atherosclerotic effect of fish consumption. Heart Vessels. 2021;36:924–933.

Zhang Z, Gao S, Dong M, Luo J, Xu C, Wen W, Huang Y, Wu Y, Zhou J, Yuan Z. Relationship between Red Blood Cell Indices (MCV, MCH, and MCHC) and Major Adverse Cardiovascular Events in Anemic and Nonanemic Patients with Acute Coronary Syndrome. Dis. Markers; 2022.

Fazio F. Fish hematology analysis as an important tool of aquaculture: A review. Aquaculture. 2019;500:237–242.

Shahsavani D, Mohri M, Kanani HG. Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiol. Biochem. 2010;36:39–43.

Abdel-Daim MM, Dawood MAO, Aleya L, Alkahtani S. Effects of fucoidan on the hematic indicators and antioxidative responses of Nile tilapia (Oreochromis niloticus) fed diets contaminated with aflatoxin B1. Environ. Sci. Pollut. Res. 2020;27:12579–12586.

Lu K le, Xu W na, Li J yi, Li X. fei, Huang G, qing Liu W bin. Alterations of liver histology and blood biochemistry in blunt snout bream Megalobrama amblycephala fed high-fat diets. Fish. Sci. 2013;79:661–671.

Keri Alhadi I, Aziz A, Abol-Munafi aB. Haematological changes in Nile tilapia (Oreochromis niloticus) fed with varying dietary maltose levels. World J. Fish Mar. Sci. 2012;4:376–381.

Baba E, Acar Ü, Öntaş C, Kesbiç OS, Yılmaz S. Evaluation of Citrus limon peels essential oil on growth performance, immune response of Mozambique tilapia Oreochromis mossambicus challenged with Edwardsiella tarda. Aquaculture. 2016;465:13–18.

Magnadóttir B. Innate immunity of fish (overview). Fish Shellfish Immunol. 2006; 20:137–151.

Metwally MAA, El-Gellal AM. Used of Some Plant Wastes for Fish Feeding with Reference on its Impact on Growth Performance and Body Composition. World Appl. Sci. J. 2009;6:1309–1313.