Monoculture and Mixed Culture of Pacific White Shrimp Litopenaeus vannamei and Tiger Shrimp Penaeus monodon in Biofloc System: A Comparative Study

Yenukolu Aparna

Department of Zoology, Division of Aquaculture, Sri Venkateswara University, Tirupati – 517502, India.

Shaik Haleema Banafsha

Department of Zoology, Division of Aquaculture, Sri Venkateswara University, Tirupati – 517502, India.

Motireddy Srinivasulu Reddy *

Department of Zoology, Division of Aquaculture, Sri Venkateswara University, Tirupati – 517502, India.

*Author to whom correspondence should be addressed.


Abstract

The microbial modification method known as Biofloc Technology (BFT) has been used regularly in prawn culture operations lately. The primary component of BFT culture systems, microbial Biofloc, is responsible for improving water quality, supplementing natural nutrition, and encouraging the growth and welfare of grown prawns. An attempt has been made to investigate the effects of adding probiotics (Bacillus licheniformis and Lactobacillus rhamnosus) and the Biofloc system on water quality parameters and the performance of shrimp Litopenaeus vannamei and Penaeus monodon. Various external carbon sources, such as molasses and tapioca flour combinations, were used for the development of Bioflocs in the present study both under monoculture and mixed culture operations. Experimental diets comprising one control, one supplemented with probiotics and bioflocs (combinations of tapioca flour and molasses developed with varying external carbon sources), and both monoculture and mixed culture operations of Litopenaeus vannamei and Penaeus monodon were used in feeding trail experiments for about 120 days. Because mixtures of both Probiotics and Bioflocs were added, all the water quality indicators remained in optimal condition and provided resistance and protection against Candidate species of cultivation.  Along with acting as a food supplement for prawns, intense mechanical aeration also promotes the microbial community's breakdown of toxic nitrogen and other pollutants. Productivity rates increased dramatically as a result. Therefore, BFT is an effective pathogen replacement, preserves ideal water quality, and boosts the growth and survival of prawns from L. vannamei and P. monodon. Probiotics and Bioflocs are used in combination in this work to maintain good water quality metrics and P. monodon and L. vannamei growth performance.

Keywords: L. vannamei, P. monodon, Bioflocs, C:N ratios, culture, penaeus monodon, microbial community's, microbial biofloc


How to Cite

Aparna , Y., Banafsha , S. H., & Reddy , M. S. (2024). Monoculture and Mixed Culture of Pacific White Shrimp Litopenaeus vannamei and Tiger Shrimp Penaeus monodon in Biofloc System: A Comparative Study. Asian Journal of Fisheries and Aquatic Research, 26(3), 87–106. https://doi.org/10.9734/ajfar/2024/v26i3749

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References

Zhang ZF, Zhang WB, Liu LP. Status and sustainability problems of white leg shrimp Litopenaeus vannamei culture in Zhanjiang (In Chinese with English abstract). Guandong Agric Sci. 2012; 39:158-60.

Jackson C, Preston NP, Thompson PJ, Burford MA. Nitrogen budget and effluent nitrogen components at an intensive shrimp farm. Aquaculture. 2003;218:397-11.

DOI:10.1016/S0044-8486(03)00014-0

Liping L, Zhenxiong H, Xilin D, Yoram A. Effects of addition of maize starch on the yield, water quality and formation of bioflocs in an integrated shrimp culture system. Aquaculture. 2014;418-419:79-86.

DOI:https://doi.org/10.1016/j.aquaculture.2013.10.005

Verdian AH, Effendi I, Budidardi T, Diatin I. Production performance improvement of white shrimp Litopenaeus vannamei culture with integrated multi trophic aquaculture system in Seribu islands, Jakarta, Indonesia. Iranian J of Fisheries Sci. 2020;1415-27.

DOI: 10.22092/ijfs.2019.120676.

Avnimelech Y. Feeding with microbial flocs by tilapia in minimal discharge bio- flocs technology ponds. Aquaculture. 2007;264: 140-47.

Sirisha K, Aparna Y, Srinivasulu Reddy M. Efficacy of Probiotics and Bioflocs on the production performance of Tiger shrimp Penaeus monodon and Pacific white shrimp Litopenaeus vannamei under polyculture operation. Asian J of Fisheries and Aqua Res. 2021;11(2):15-28.

DOI: 10.9734/ajfar/2021/v11i230198

Avnimelech Y. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture. 1999;176(3):227-35.

DOI:10.1016/S0044-8486(99)00085-X

Hargreaves JA. Photosynthetic suspended-growth systems in aquaculture. Aquacul Engi. 2006;34:344-63.

Burford MA, Thompson PJ, McIntosh RP, Bauman RH, Pearson DC. The contribution of flocculated material to shrimp (Litopenaeus vannamei) nutrition in a high-intensity, zero-exchange system. Aquaculture. 2004;232:525-37.

DOI: 10.1016/S0044-8486(03)00541-6

Krummenauer D, Abreu PC, Poersch LH, Paiva Reis PAC, Suita SM, Reis WG, Wasielesky W. The relationship between shrimp (Litopenaeus vannamei) size and biofloc consumption determined by the stable isotope technique. Aquaculture. 2020;529(83):735635.

DOI:10.1016/j.aquaculture.2020.735635

Naresh S. Efficacy of selected Probiotics and Prebiotics in larval Rearing and culture of Penaeid prawns Penaeus monodon and Penaeus semisulcatus. Ph.D Thesis. Sri Venkateswara University. Tirupati; 2016.

Avnimelech Y, Mokady S, Schroeder GL. Circulated ponds as efficient bioreactors for single-cell protein production. Israeli J of Aquacult Bamidgeh. 1989;41:58-66.

Avnimelech Y, Diab S, Kochva M, Mokady S. Control and utilization of inorganic nitrogen in intensive fish culture ponds. Aquacult Res. 1992;23(4):421-30.

DOI:https://doi.org/10.1111/j.1365-2109.1992.tb00786.x

Avnimelech Y, Kochva M, Hargreaves JA. Sedimentation and Resuspension in Earthen Fish Ponds. J of the World Aquacult Soc. 1999;30(4):401-09.

DOI:10.1111/j.1749-7345.1999.tb00988.x

Ekasari J, Crab R, Verstraete W. Primary nutritional content of Bioflocs cultured with different organic sources and salinity. Hayati J of Biosci. 2010;17(3):125-30.

DOI: https://doi.org/10.4308/hjb.17.3.125

Walkley A, Black IA. An examination of the Degrjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci. 1934;37:29-38.

DOI: http://dx.doi.org/10.1097/00010694-193401000-00003

APHA. Standard Methods for the examination of Water and Waste water. 19th ed. American Public Health Association, New York; 2005.

Sirisha K. Efficacy of Probiotics and Bioflocs on the culture operation of white shrimp Litopenaeus vannamei. Ph.D Thesis, Sri Venkateswara University, Tirupati; 2022.

AOAC. Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists; Arlington, VA, USA; 2005.

Ferreira NC, Bonetti C, Seiffert WQ. Hydrological and Water Quality Indices as management tools in marine shrimp culture. Aquaculture. 2011;318(3-4):425-33.

DOI:https://doi.org/10.1016/j.aquaculture.2011.05.045

Abdo Hassan Sh, Helal AM, El-Darawany AA, Salah AM. Effects of poly culture shrimp, Litopenaeus vannamei with keeled mullet, Liza carinata at different densities compared to the monoculture system on physico-chemical water quality. Zagazig J of Agricul Res. 2021;48(3):817-27.

Hoang MN, Nguyen PN, Bossier P. Water quality, animal erformance, nutrient budgets and microbial community in the biofloc-based polyculture system of white shrimp, Litopenaeus vannamei and gray mullet, Mugil cephalus. Aquaculture. 2020;515:734610.

DOI:https://doi.org/10.1016/j.aquaculture.2019.734610

Ritvo G, Dixon JB, Lawrence AL, Samocha MT, Neil WH, Speed MF. Accumulation of chemical elements in Texas shrimp pond soils. J of the World Aquacul Soc. 1998;29:422-31.

Khanjani MH, Alizadeh M, Sharifinia M. Effects of different carbon sources on water quality, biofloc quality, and growth performance of Nile tilapia (Oreochromis niloticus) fingerlings in a heterotrophic culture system. Aquacult International. 2021;29:307-21.

DOI: 10.1007/s10499-020- 00627-9.

Carbajal Hernandez JJ, Fernandez LPS, Villa LA, Ochoa JA, Trinidad JFM. Water quality assessment in shrimp culture using an analytical hierarchical process. Ecological Indiators. 2013;29: 148-58.

DOI: 10.1016/j.ecolind.2012.12.017

Ebeling JM, Timmons MB, Bisogni JJ. Engineering analysis of the stoichiometry of photoautotrophic, autotrophic and heterotrophic removal of ammonia-nitrogen in aquaculture systems. Aquaculture. 2006;257(1-4):346-58.

DOI:https://doi.org/10.1016/j.aquaculture.2006.03.019

De Schryver P, Crab R, Defoirdt T, Boon N, Verstraete W. The basics of bioflocs technology: the added value for aquaculture. Aquaculture. 2008;277(3-4):125-37.

DOI:https://doi.org/10.1016/j.aquaculture.2008.02.019

Krishna Naik M, Srinivasulu Reddy M. Performance of shrimp Litopenaeus vannamei with the addition of Probiotics and Bioflocs; A field study. Int J of Fisheries & Aquatic studies. 2020; 8(3):286-91.

Lalitha Kumari Ch. The Effect of Bioflocs on the Aquaculture production with special emphasis to Pacific White shrimp Litopenaeus vannamei. PhD Thesis S.V University, Tirupati, India; 2019.

Alexander C, Akhtar MS. Nitrite implications and its management strategies in aquaculture: a review. Reviews in Aquacul. 2020;12(2):878-08.

DOI: https://doi.org/10.1111/raq.12354

Santhosh Kumar, Verma AK, Singh SP, Awasthi A. Immunostimulants for shrimp aquaculture: paving pathway towards shrimp sustainability. Environ Sci and Pollution Res. 2023;30:25325-343.

Gladys VC, Martin GFE, Cecilia V, Cristina CS. Toxicity of Ammonia, nitrite and nitrate to Litopenaeus vannamei juveniles in low-salinity water in single and ternary exposure experiments and their environmental implications. Environ Toxicol and Pharmacol. 2019;70.

DOI:10.1016/j.etap.2019.05.002

Glen RRP, Teresa G, Luis RMC, Anselmo MB, Francisco VA, Marcel MP. The Nitrification process for nitrogen removal in Biofloc system aquaculture. Reviews in Aquacult. 2020;12(4):12431.

DOI:10.1111/raq.12431

Pankaj Kumar, Jetani KL, Yusuzai SI, Sayani AN, Dar SA, Rather MA. Effect of sediment and water quality parameters on the productivity of coastal shrimp farm. Advances in Applied Sci Res. 2012; 3(4):2033-41.

Avnimelech Y. Biofloc Technology. A Practical Guide Book. World Aquaculture Society. LA, USA; 2015.

Boyd CE, Thunjai T, Boonyaratpalin M. Dissolved salts in water for inland low salinity shrimp culture. Global Aquacult Advocate. 2002;5:40-45.

Wu JX, Lu QP, Xiao HS, Jie H. Effects of bioflocs on water quality, and survival, growth and digestive enzyme activities of Litopenaeus vannamei (Boone) in zero‐water exchange culture tanks. Aquacult Res. 2013;44(7).

DOI:10.1111/j.1365-2109.2012.03115.x

Rouf MA, Islam MMM, Hambrey J, Telfer T. Nutrient budget of Shrimp Culture Ponds In Southwest Coastal Region Of Bangladesh. J of Aquacult in the Tropics. 2012;27(1-2):41-51.

Hassan AH, Rashid T, Magreth K. Study on the water quality parameters in semi-intensive coastal shrimp culture system in Mafia Island, Tanzania. J of Environ and Earth Sci. 2015;5(1):142-50.

Farzanfar A. The use of probiotics in shrimp aquaculture. FEMS Immunol and Medical Microbiol. 2006;48(2):149-58.

Goh JXH, Tan LTH, Law JWF, Khaw KY, Zengin G, Chan KG, Letchumanan V, Lee LH, Goh BH. Probiotics: Comprehensive exploration of the growth promotion mechanisms in shrimps. Progress in Microbes and Molecular Biol. 2023;6(1):1-86.

DOI:https://doi.org/10.36877/pmmb.a0000324

Soto JO. Feed intake improvement, gut microbiota modulation and pathogens control by using Bacillus species in shrimp aquaculture. World J of Microbiol and Biotechnol. 2021;37(2):28.

DOI: 10.1007/s11274-020-02987-z.

Krishna Naik M. Monitoring The Effects of Probiotics and Bioflocs on Growth Performance and Immune Responses in Pacific White shrimp Litopenaeus vannamei. Ph.D Thesis. Sri Venkateswara University, Tirupati; 2021.

Seenivasan C, Bhavan PS, Radhakrishnan S. Effect of Probiotics (Binifit M) on survival, growth, biochemical constituents and energy budget of the fresh water prawn Macrobrachium rosenbergii postlarvae. Aquaculture. 2011;41:5919-27.

Qiu Z, Xu Q, Li S, Zheng D, Zhang R, Zhao J, Wang T. Effects of probiotics on the Water quality, Growth performance, Immunity, Digestion and Intestinal flora of Giant fresh water prawn Macrobrachium rosenbergii in the Biofloc culture system. Water. 2023;15(6):1211.

DOI: https://doi.org/10.3390/w15061211

Hasan NA, Haque MM, Hinchliffe SJ, Guilder J. A sequential assessment of WSD risk factors of shrimp farming in Bangladesh: Looking for a sustainable farming system. Aquaculture. 2020;526: 735348.

DOI: 10.1016/j.aquaculture.2020.735348

Hai NV. The use of Probiotics in Aquaculture. J of Applied Microbiol. 2015;119(4):917-35.

DOI: https://doi.org/10.1111/jam.12886

Aisyah, Ayi Y, Yuli A. Evaluation of various sources of bacteria on Biofloc productivity. Asian J of Fisheries and Aquatic Res. 2021;12(2):30-39.

DOI: 10.9734/AJFAR/2021/v12i230231

Vincent MS, Amoah K, Cai J. Effect of Bacillus Probiotics on the Immunological Responses of Nile Tilapia (Oreochromis niloticus): A Review. Fishes. 2023;8 (7):366.

DOI: https://doi.org/10.3390/fishes8070366

Kuebutornye FKA, Abarike ED, Sakyi ME, Lu Y, Wang Z. Modulation of nutrient utilization, growth, and immunity of Nile Tilapia, Oreochromis niloticus: The role of Probiotics. Aquacult International. 2020; 28(1):277-91.

DOI: 10.1007/s10499-019-00463-6

Sundaram M, Panigrahi A, Ganesh J, Nila rekha P, Sivagnanam S, Rajamanickam S, Gopal C. Evaluation of different Probiotic strains for growth performance and immuno modulation in Pacific white shrimp Penaeus vannamei Boone, 1931. Indian J of Fisheries. 2017;64:89-96.

DOI:10.21077/ijf.2017.64.special-issue.76210-13

Yenukolu Aparna, Shaik Haleema Banafsha and Motireddy Srinivasulu Reddy. Efficacy of probiotics and biofloc system in maintenance of water quality and growth performance in shrimp Litopenaeus vannamei. International Journal of Fisheries and Aquatic Studies 2023;11(6):07-20