Effects of Flour Extract of Leaves of Terminalia catappa (L) on Aeromonas Motile Septicemia in Cyprinus carpio (L) Larvae
Adilah Nuryani
Fish Hatchery Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia.
Ardiansyah *
Fish Hatchery Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia.
Amrullah
Fish Hatchery Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia.
Indrayani
Agricultural Technology Education Study Program, Faculty of Engineering Makassar State University, Indonesia.
Dahlia
Fish Hatchery Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia.
Rusli
Aquaculture Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to evaluate the effectiveness of Terminalia catappa (L) leaf flour extract in controlling Motile Aeromonas Septicemia (MAS) disease caused by A. hydrophila in common carp larvae. The research method used in this study is an experimental method using a complete randomized design (RAL). This research consists of 2 stages; in vitro and in vivo tests using T. catappa leaf extract. In vitro test, T. catappa leaf extract consists of 5 different doses; The treatments consisted of 0 (negative control), 20, 40, 80, and 100 g L-1 T. catappa leaf flour extract and Stroptomycinsulphate 0.005 g (positive control). In vivo test, 3 best doses were selected based on the diameter zone of inhibition; 60, 80, and 100 g L-1, with positive (using Stroptomycinsulphate 0.005 g and untreated sample (without T. catappa leaf flour extract), each with 3 replicates. The results showed that using T. catappa leaf flour extract can significantly increase the fish's immune response, resistance, and survival. Duncan's further test showed that the 100 g L-1 dose treatment revealed an increase in immune response and survival rate compared to the F and G treatments.
Keywords: Erythrocytes, leukocytes, hemoglobin, hematocrit, phagocytosis index, survival rate