Open Access Original Research Article

A Study on Some Biological Aspects of Giant Snakehead Fish (Channa micropeltes) in Huai Suea Ten Wetland Site in Thailand

Kampon Thaiso, Bundit Yuangsoi, Sutee Wongmaneeprateep, Pornthep Niamphithak, Pattama Wiriyapattanasub, Siriporn Tola, Siripavee Charoenwattanasak

Asian Journal of Fisheries and Aquatic Research, Page 1-10
DOI: 10.9734/ajfar/2019/v3i330033

This research was aimed to study on biological aspects of giant snakehead fish (Channa micropeltes) in Huai Suea Ten wetland site, Nam Phong District, Khon Kaen Province in Thailand between April, 2017 and March, 2018. Thirty-six fish species belonging to 17 families were observed at 10 sampling sites. Three Channidae fishes, namely snakehead fish (Channa striata), giant snakehead fish (Channa micropeltes), and forest snakehead fish (Channa lucius), belonging to the family Channidae were reported. A total of 230 giant snakehead fish were found in this survey which consisted of 118 males and 112 females. The sex ratio (male: female) was 1:0.95. The gonadosomatic index value of the female (0.79±0.05%) was higher than that of the male (0.25±0.08%). The body length and body weight of a total of 230 giant snakehead fish observed in this study showed the average of a total body length was 43.71±11.72 cm; and the average of the body weight was 448±173 g/fish. The condition factor of giant snakehead fish observed in 12 months were in a range of 0.45-0.74 for the male and 0.39-0.70 for the female. The ratio of the body and intestinal length of giant snakehead fish was as 1: 0.70. The stomach digesta components, expressed as the percentages of a whole stomach content weight of this fish species consisted of 51.8% of fish fillet, 30.3% of small fish and 17.9% of digested digesta.

Open Access Original Research Article

Evaluation of the Antioxidant Markers in Clarias gariepinus Heart Exposed to Mercury and Ammonium Chloride

A. S. Eboh, E. M Arhoghro

Asian Journal of Fisheries and Aquatic Research, Page 1-6
DOI: 10.9734/ajfar/2019/v3i330034

Reactive Oxygen Species (ROS) parameters and antioxidant enzyme activities in heart of Clarias gariepinus weighing (15 ± 1.7 g) in toxic exposure to mercury concentrations, 0.1 mg/ L group B, group C 10 mg/L ammonium chloride and group D 0.1 mg/L mercury and 10 mg/L ammonium chloride for 7 consecutive days at 26 ± 1°C temperature. Group A was exposed to water alone and serve as control. At the end of the experimental period, Reduced glutathione (GSH), NO levels and the activities of Peroxidase, Superoxide dismutase (SOD) and Alanine Amino Transferase (ALT) in heart were assayed. The result showed a significant increase (P <0.05) in the activities of ALT, Peroxidase, and SOD. However, a significant decrease in the levels of total protein content, GSH and NO were also observed. The results showed that the combined effect of mercury and ammonium had an adverse effect greater than that obtained individually.

Open Access Original Research Article

Optimum Fertilizer Media and Plant Substrates: (Musa paradisiaca L. Peels, Carica papaya L. Peels and Brassica oleracea var. capitata L. Leaves) for the Growth of Freshwater Rotifer (Brachionus calyciflorus Pallas, 1766)

L. E. Igoche, O. F. Okafor, P. C. Ofojekwu, G. A. Ruma

Asian Journal of Fisheries and Aquatic Research, Page 1-6
DOI: 10.9734/ajfar/2019/v3i330035

The aim of this study was to search for a cheap source for the mass production of Brachionus calyciflorus Pallas, 1766 which is a good substitute for artemia in the culture of C. gariepinus hatchlings. Poultry manure (1.25 g/L), soya bean (1.25 g/L), groundnut cake (1.25 g/L) and single super phosphate (0.04 g/l) were used exclusively and in combinations as fertilizer media in the culturing of Brachionus calyciflorus Pallas, 1766 used in feeding C. gariepinus hatchlings. The four fertilizer media were combined to give four treatments with two replicates. Three fresh plant substrate (Carica papaya L. Peels, Musa paradisiaca L. peels and Brassica oleracea var. capitata L. leaves) were introduced into treatments labeled A (Poultry manure, soya bean, groundnut cake, single super phosphate and banana pills), B (Poultry manure, soya bean, groundnut cake, single super phosphate and Carica papaya L. Peels), C (Poultry manure, soya bean, groundnut cake, single super phosphate and cabbage slice) with D(Poultry manure, soya bean, groundnut cake, single super phosphate) as the control. A total of 20 Individuals/mL of Brachionus calyciflorus Pallas, 1766 were inoculated into each treatment and the culturing lasted for 12 days. At the end of the culturing period, the best doubling time (tD) were observed in treatment A and B with a doubling time of 2.89 on the 10th day.

Result of the study showed medium B and C to be sig. different (P< 0.05) and performing better than A and D in the early stages of the study. There was an initial lull in production using the C medium, as it had the least performance. By day eight, it had picked up and there was no sig. difference (P>0.05) in production of B. calyciflorus Pallas, 1776 using the four culture media. Thus any of the media can be used for mass production of B. calyciflorus. Use of media B and C is however, recommended because they had a higher carrying capacity and can sustain production over a longer period using a giving quantity compared to the other two media.

Open Access Original Research Article

Impact of Pollution on Haematology and Histology of Juveniles of Chrysichthys nigrodigitatus in Ogbese River, Ondo State, Nigeria

Abidemi-Iromini, Atilola Olateju

Asian Journal of Fisheries and Aquatic Research, Page 1-12
DOI: 10.9734/ajfar/2019/v3i330036

The silver catfish Chrysichthys nigrodigitatus is of economic importance in sub-sahara Africa. In Ogbese town, and its environs, it constitutes a means of income and food for fisherfolks and community members. Hence, this study was undertaken to assess health status of Chrysichthys nigrodigitatus using haematology and histological assessment of the fish specie due to the anthropogenic activities that takes place around the river body.  A total 120 live juvenile fish samples of C. nigrodigitatus were collected around shallow habitats of Ogbese River by the assistance of fisherfolks using fish cage. Some water parameters measurements were taken: temperature, pH, DO, Turbidity and Conductivity. Morphometric measurement: Weight (g) and length (cm) of fish were taken. Haematology and histology of fish gills, liver and intestine were determined. Mean water temperature (27.70±0.18°C), pH (7.36±0.22), DO (6.98±0.15 mg/l), Turbidity (78.50±13.53 NTU) and Conductivity (148.35±27.98) of the river determined respectively. Mean body weight of fish was 148.15 ± 36.53 g, and mean length was 25.64 ± 2.86 cm.  The gills, liver and intestines of the fish specie were examined to assess the architecture of the organs. Results of haematology studies of C. nigrodigitatus revealed high values in the parameters measured. Red Blood Cell was higher than the White Blood Cell with mean value of (225.63±10.45 103/mm3) while Eosinophils recorded lowest parameters with mean value of (1.75±0.52%). Results of histology of gills, liver and intestines showed that the gill filaments were eroded with deformation of the cartilage core and also hyperplasia of the secondary lamellae. The intestines showed atrophy in a mucosal layer, hemorrhage and dilation within blood vessels and within serosa of mucosa and for liver, picnotic nucleus were shattered, the hepatocytes were ruptured and there was increased kupffer cell count as a result of exposure to pollutants. The results indicated that pollution level of the environment have significant impact on health status of fish.

Open Access Original Research Article

Efficacy of Aloe vera Gel and Water-leaf Extracts for Removal of Egg Adhesiveness during Artificial Propagation of African Catfish (Clarias gariepinus, Burchel 1822)

S. J. Fawehinmi, O. T. Adebayo, O. K. Gbadamosi

Asian Journal of Fisheries and Aquatic Research, Page 1-13
DOI: 10.9734/ajfar/2019/v3i330037

The paper aims to study the best immersion period and concentration of the plant extracts under study (water-leaf and Aloe vera gel) that can efficiently remove egg adhesiveness of Clarias gariepinus. Data generated were subjected to Multivariate Analysis of Variance Test, Tukey multiple range tests was used as a follow up procedure. Third order Polynomial regression analysis was then used to determine the best concentration and immersion period that neutralizes adhesiveness in eggs of C. gariepinus. The experiment was carried out at The Teaching and Research Fish Farm, Federal University of Technology, Akure, between October 2018 to December 2018. One male and one female C. gariepinus brood stock weighing 1.0 kg and 1.3 kg, respectively were used for the experiment. Three different concentrations (1, 3, and 5)% of the plant extracts were used. 2 g of urea diluted in 4 g NaCl/L of water was used as reference de-adhesion agent, while water without urea solution nor plant extracts was used as control. The fish eggs were rinsed with the solutions at different durations of 1, 3 and 5 minutes. Each concentration and rinsing time was recorded in duplicates. Results of the study showed that there were no significant differences (p=0.05) in the non-adhesive egg hatching of eggs immersed in Aloe vera gel and Urea solution. Number of non-adhesive egg and hatching increased in waterleaf extract which was significantly different (P=0.05) from eggs immersed in Aloe vera gel and water but not significantly different from urea solution. Therefore this study shows that waterleaf extract at 1% concentration and 1 minute immersion period can efficiently remove egg adhesiveness and increase hatching in C. gariepinus.