Open Access Original Research Article

Effect of Adding Fermented Restaurant Waste Meal with Different Concentration to Physical Quality of Fish Pellet

Meri Alex Sandra, Yuli Andriani, Kiki Haetami, Walim Lili, Irfan Zidni, Muhammad Fatah Wiyatna

Asian Journal of Fisheries and Aquatic Research, Page 1-7
DOI: 10.9734/ajfar/2019/v5i330074

This study aims to evaluate the physical quality of fish feed pellets formulated with different concentration of fermented restaurant waste meal (FRWM). The treatments given consisted of treatment A (control, without adding FRWM), treatment B (adding FRWM 10%), treatment C (adding FRWM 20%), treatment D (adding FRWM 30%) and treatment E (adding FRWM 40%). The study was conducted from July to October 2019 at the Aquaculture Laboratory and Hatchery in Ciparanje of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The parameters observed include the breaking rate test, sinking rate test, durability test, and stability test. Results showed that the use of FRWM given significant difference (p<0.05) for breaking rate and stability value. However, result showed that the sinking rate and durability value generally did not show significant difference (p>0.05). The FRWM can be used up to a concentration of 30% which gives effect to the sinking rate of the fish pellet which is similar to the control fish pellet namely 0.38 cm/s while the concentration of the meal of the FRWM gives the closest value of breaking rate and stability control as much as treatment B (adding FRWM 10%) with a break rate for 19.93 hours and stability in water for 10 minutes by 91.66%, 30 minutes by 87.60%, and for 60 minutes by 75.26%. The different adding concerntration of FRWM gives effect to the quality of fish pellet while more than 10% can decrease its quality. The difference were show in all treatment in some parameters observed.

Open Access Original Research Article

A Comparison between Chicken Viscera and Housefly Maggot Cultured from this By-products for Nile Tilapia Diets: Growth Performance, Feed Utilization and Whole Body Composition

Cayen S. Alofa, Youssouf Abou

Asian Journal of Fisheries and Aquatic Research, Page 1-12
DOI: 10.9734/ajfar/2019/v5i330075

Aims: The feeding trials were conducted to compare the effects of partial fishmeal replacement by two different animal protein sources on growth performance, feed utilization efficiency and body composition of juvenile Nile tilapia.

Place and Duration of Study: The study was carried out at the Aquaculture Research Center, Department of Zoology, Faculty of Sciences and Technics, University of Abomey-Calavi for a period of 84 days.

Methodology: Three isonitrogenous and isoenergetic diets were formulated containing fishmeal (CD), chicken viscera meal (CVM) and housefly maggot meal (HMM), as partial fishmeal replacement. Commercial diet Skretting (SK) was used as reference diet. All male Oreochromis niloticus with an average initial body weight of 8.65 g were fed thrice a day to apparent satiation. Each treatment was randomly attributed to three replicates.

Results: Fish fed all experimental diets showed no effects (P>.05) on survival rate (91.33-96.00 %), condition factor (1.85-1.9), protein efficient ratio (2.05-2.37) and feed conversion ratio (1.21-1.40), although higher values were observed with control diets. Final mean weight and daily weight gain of fish fed HMM diet (88.31 g ; 0.95 g. days-1) were not significantly different from those fed control diets C (88.54 g; 0.95 g/j) and Sk (87.59 g; 0.94 g/j) respectively. Growth performances significantly decreased (P<.05) in CVM group (75.09 g; 0.75 ± g/j). Whole-body protein contents were similar in all groups, whereas lipid content was highest in those fed CVM.

Conclusion: The results indicated that 200 g fishmeal per kilo diet can be successfully replaced with 250 g.Kg-1 of HMM without adverse effect on growth and feed utilization whereas CVM inclusion did not perform also well. 

Open Access Original Research Article

Utilization of Aquatic Plants as Phytoremediation Agents of Tofu Liquid Waste

Qulsum C. Dewi, Zahidah Hasan, Herman Hamdani, Heti Herawati

Asian Journal of Fisheries and Aquatic Research, Page 1-9
DOI: 10.9734/ajfar/2019/v5i330076

The tofu industry produces a lot of tofu liquid waste and has the potential to cause waters to be toxic and dangerous for the life of the biota aquatic. This research uses a phytoremediation method using floating water plants. The purpose of this research was to obtain water plants that have better absorption ability as phytoremediation agents for tofu liquid waste between Lemna and Kiapu aquatic plants. This research was conducted at the Laboratory of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Padjadjaran University from March to September 2019. The method used in this research was an experimental method with a Completely Randomized Design (CRD) consisting of three treatments and five replications. Tofu liquid waste samples were obtained from the Tofu Factory in the Cibuntu Tofu Industry Center. Physical and chemical parameters observed include temperature, acidity, dissolved oxygen, BOD5, nitrate, and phosphate. The results showed that the Kiapu had better nitrate and phosphate absorption ability than Lemna and the combination treatments for 15 days of observation. The result showed that the decrease in nitrate concentration was 10.12-62.24% and the decrease in phosphate concentration was 6.67-54.23%. Kiapu can be recommended as phytoremediation agents for tofu liquid waste to remediate high organic matter before being discharged directly into water bodies.

Open Access Original Research Article

Microbial Diversity Assessment in Milkfish Culture Ponds

L. M. M. Dalmacio, B. L. Ramirez, R. Estacio, I. Borlongan, J. M. Ramirez, K. V. Evangelista, E. Madlangbayan, F. Guillergan, M. A. Kron

Asian Journal of Fisheries and Aquatic Research, Page 1-12
DOI: 10.9734/ajfar/2019/v5i330077

Aims: To determine bacterial diversity in milkfish culture ponds that contain different life-cycle stages of the milkfish (pond A: fry, pond B: juveniles and pond C: adults) by DNA sequence analysis of organisms and compare that microbial diversity to organisms found in soil adjacent to the ponds.

Study Design: Comparative metagenomic study of aquatic and terrestrial biodiversity based on DNA sequence analysis of water and soil DNA.

Place and Duration of Study: SEADEC milkfish ponds in Tingnauan, Iloilo. Philippines. All water and soil samples were collected over a three-day period.  

Methodology: DNA sequence analysis of nucleic acids extracted from water samples collected from the three types of milkfish ponds along with soil adjacent to the ponds. DNA was extracted and PCR was performed using the 11F-1492R primer pair to amplify 16S rRNA gene. Purified 16S rDNA amplicons were cloned in using the TOPO-TA cloning kit for DNA sequencing. 16s rRNA gene sequences were analyzed with the use of software tools at the National Center for Biotechnology Information website and imported into the ARB phylogenetic analysis software. Distance matrices were exported using the neighbor-joining algorithm in ARB, in the form of PHYLIP-formatted lower triangular matrices. The distance matrices were then used to calculate Shannon-Weaver and Simpson diversity indices to evaluate the richness and evenness of the sampled populations. Rarefaction curves were determined to evaluate sampling efficiency.

Results: Rarefaction curves indicated that the sampling effort was sufficient to reveal the majority of phyla present in the sample. Shannon-Weaver and Simpson indices suggested that the diversities of all the groups were statistically different from each other. It was observed that pond A was least diverse, followed by pond C and pond B. The soil was most diverse. DNA sequence analysis identified the various species of bacteria in soil and water.

Conclusion: All three pond communities were significantly different in diversity. This study did not identify any significant human pathogens such as Vibrios, Salmonella or Shigella. Bacterial diversity of sites decreased in the following order: soil > fry pond > fingerling pond > adult pond. 

Open Access Original Research Article

The Use of Various Proportions of Rough Fish on Giant Trevally (Caranx sp.) Culture in the Floating Net Cages, Pangandaran, West Java

Rita Rostika, Muhamad Ihsan Fauzan, Walim Lili, Iskandar .

Asian Journal of Fisheries and Aquatic Research, Page 1-7
DOI: 10.9734/ajfar/2019/v5i330079

This research aims to determine the effect of providing rough fish and pellets with optimal dosages on the Giant Trevally culture to produce the highest growth in Pangandaran floating net cages. This research was conducted in August 2019 to September 2019 with 60 days fish rearing. The method used in this study was a Completely Randomized Design (CRD) with five treatments and three replications. The treatments were A (0% rough fish and 100% pellets), B (25% rough fish and 75% pellets), C (50% rough fish and 50% pellets), D (75% rough fish and 25% pellets) and E (100% rough fish and 0% pellets). The effect of each treatment was tested by analysis of variance (ANOVA) F test at an interval of 5%; if there were significant differences then followed by Duncan's multiple range test. The parameters observed in this study included fish survival (SR), Daily Growth Rate (LPH), Feed Efficiency (EPP) and water quality at the study site. The results obtained from this study were the provision of rough fish in Giant Trevally was able to increase the Daily Growth Rate (LPH) and the Efficiency of Feeding (EPP) for Giant Trevally. The highest growth value in this study was in treatment E (with 100% rough fish and 0% pellets) with LPH of 3.01%, total weight of 109.47gr and EPP of 30.60%.